BME100 s2014:W Group2 L5: Difference between revisions
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Instructor's summary: You completed 8 PCR reactions in a previous lab. You used the SYBR Green I staining and imaging technique to measure the amount of amplified DNA in each PCR reaction. You used a standard curve (based on known concentrations of calf thymus DNA) to convert INTDEN values into DNA concentration. Your positive control and negative control samples should be used as threshold values for determining whether an unknown (patient) sample is truly positive or negative. | Instructor's summary: You completed 8 PCR reactions in a previous lab. You used the SYBR Green I staining and imaging technique to measure the amount of amplified DNA in each PCR reaction. You used a standard curve (based on known concentrations of calf thymus DNA) to convert INTDEN values into DNA concentration. Your positive control and negative control samples should be used as threshold values for determining whether an unknown (patient) sample is truly positive or negative. | ||
Your positive control PCR result was: | Your positive control PCR result was: 12.11 μg/mL | ||
Your negative control PCR result was: | Your negative control PCR result was: 0.75 μg/mL | ||
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Compare each patient's results to the positive control value and the negative control value. Draw a final conclusion for each patient (positive or negative) and explain why you made that conclusion. | Compare each patient's results to the positive control value and the negative control value. Draw a final conclusion for each patient (positive or negative) and explain why you made that conclusion. | ||
Patient 51225 : The patient's sample turned out to be positive because of the green color given off. | Patient 51225 : The patient's sample turned out to be positive because of the green color given off. Indicating that the patient does not have the disease. | ||
Patient 60779 : The patient's sample turned out to be negative because of the blue color given off. | Patient 60779 : The patient's sample turned out to be negative because of the blue color given off. Indicating that the patient has the disease. | ||
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Latest revision as of 09:55, 16 April 2014
BME 100 Spring 2014 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAM
LAB 5 WRITE-UPBackground InformationSYBR Green Dye Single-Drop Fluorimeter How the Fluorescence Technique Works
ProcedureSmart Phone Camera Settings
Adjust the distance between the smartphone on its cradle and the first two rows of the slide so that it is as close as you can get without having a blurry image. Record the distance between the smart phone cradle and drop using the ruler provided.
Solutions Used for Calibration
Data AnalysisRepresentative Images of Samples
Image J Values for All Samples [Instructions: See worksheet page 8. To save time on typing a new Wiki table from scratch, use THIS TOOL to auto-generate a Wiki table: Excel-to-Wiki Converter. Copy the headers and values from the Excel spreadsheet you made, paste them into the form field, click submit, copy the Wiki code that the tool generated, and replace TABLE GOES HERE (below) with your auto-generated code.]
Fitting a Straight Line PCR Results Summary
Your positive control PCR result was: 12.11 μg/mL Your negative control PCR result was: 0.75 μg/mL
Write-in each patient ID and give both a qualitative (what the images looked like) and a quantitative description (μg/mL) of what you observed Patient 51225 : We pbserved a green color for the patient. Indicating the samples were positive. Patient 60779 : We observed a blue color for the patient. Indicating the samples were negative. Compare each patient's results to the positive control value and the negative control value. Draw a final conclusion for each patient (positive or negative) and explain why you made that conclusion. Patient 51225 : The patient's sample turned out to be positive because of the green color given off. Indicating that the patient does not have the disease. Patient 60779 : The patient's sample turned out to be negative because of the blue color given off. Indicating that the patient has the disease.
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