BME100 s2014:T Group9 L6: Difference between revisions
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<br> | <br> Our design will be a software change versus a physical change to the PCR machine. This design of the machine is solid. It is comprised of affordable materials, but they are durable and reliable. However, the software is extremely simple and seems to lack something. Our idea is to add a real-time video of what is actually happening within the machine. It is not going to be a microscopic view of the actually reaction, but it will be a pre-made video that will show the steps of the PCR as it goes through each cycle. This will allow teachers to help students learn what is actually happening within the machine. It will also help students understand how much DNA is actually being replicated per cycle. | ||
Revision as of 10:19, 24 April 2014
BME 100 Spring 2014 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | ||||
OUR COMPANY
LAB 6 WRITE-UPComputer-Aided DesignTinkerCAD TinkerCAD is a straightforward and easy to use program that allows the user to create different visual projects. The user can select from premade shapes the program offers, like spheres or cubes, and warp them in different sizes. It is also possible to change the color and combined many shapes into the desired pictures. TinkerCAD was used in this lab to effectively show the design being presented by this group.
Feature 1: Disease SNP-Specific PrimersBackground on the disease-associated mutation It was found the species for this variation is homo sapien. This variation is found on teh chromosome 6:149721690 and the clinical significance was listed as other. This SNP is associated with the genes SUMO4 and TAB2. SUMO4, small ubiquitin-like modifier 4, is located in the cytoplasm of cells and specifically modifies IRBA. The diseases linked to it are type 1 Diabetes, Type 2 Diabetes, nephropathy, and VKH syndrome.
Primer design
If the template contains the non-disease allele PCR should not occur. This is due to the disease-specific primer and template not binding 1oo% to the non-disease allele because the non-disease allele had a sequence of GTG, when the disease-assocoated allele has a sequence of ATG.
Feature 2: Consumables KitThe consumables kit will be held within a small b ox. This will include all the materials needed to preform a small number of PCR reactions. All items will be packaged in groups based on the item. They will then be labeled with a short description, and then they will be placed in the consumables box. This includes
Feature 3: Hardware - PCR Machine & FluorimeterIn order to create an easier teaching environment, the current PCR design has been revamped to include a clear lid. The lid will consist of both glass and plastic. The glass will be facing the samples on the inside of the lid in order to keep the samples safe from outside contaminants and regulate the temperature, and heavy duty plastic will surround the glass to give more stability to the machine.
Bonus Opportunity: What Bayesian Stats Imply About The BME100 Diagnostic ApproachWhen looking into the PCR results, it was made clear that the machine did not do the best job at predicting illness. For the percentage of people who developed the disease and also received a positive result from the machine was not a strong percentage. Unfortunately, it was not close to one, like it ideally would be. On the other hand, the people who did not develop the illness and received a negative result was closer to 1 than the prior results. While that is a much stronger result, overall the machine does not have a strong reliability. |