BME100 s2014:T Group8 L5: Difference between revisions
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Write-in each patient ID and give both a qualitative (what the images looked like) and a quantitative description (μg/mL) of what you observed <br> | Write-in each patient ID and give both a qualitative (what the images looked like) and a quantitative description (μg/mL) of what you observed <br> | ||
Patient | Patient 1 low dna concentrations didn't visibly show green, but as concentration increased, green increased. : <br> | ||
Patient 2 all of their drops showed little to no green dye. : <br> | Patient 2 all of their drops showed little to no green dye. : <br> | ||
Compare each patient's results to the positive control value and the negative control value. Draw a final conclusion for each patient (positive or negative) and explain why you made that conclusion. <br> | Compare each patient's results to the positive control value and the negative control value. Draw a final conclusion for each patient (positive or negative) and explain why you made that conclusion. <br> | ||
Patient 1 positive : <br> | Patient 1 positive, it's similar to the positive control value, and was very green.: <br> | ||
Patient 2 negative : <br> | Patient 2 negative, was blue, had little to no green, far from the positive control value. : <br> | ||
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Revision as of 01:18, 17 April 2014
BME 100 Spring 2014 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAM
LAB 5 WRITE-UPBackground InformationSYBR Green Dye
The way the fluorescence works is by utilizing a green dye that activates only under the presence of double stranded DNA (or dsDNA).
ProcedureSmart Phone Camera Settings
The camera phone was set up in the cradle with an angle to view the side of the drop. The fluorimeter was raised about 1 1/4 inches to accomodate for the phone height.
The phone was removed from this image to simplify and completely show distance setup.
Solutions Used for Calibration
Data AnalysisRepresentative Images of Samples High DNA - using green channel
Fitting a Straight Line Intends values for each Calf Thymus DNA cocnentration. PCR Results Summary A Polymerase Chain Reaction (PCR) was completed for 8 DNA samples--3 DNA samples from each of two patients and a positive and a negative control sample. After the reactions and cool down, the samples were tested on a fluorimeter for amplification of DNA using SYBR Green I as a stain. The controls were necessary to ensure amplification results for the patients' samples had a comparative standard and the results were due to the procedure and not an external affect. Using known concentration of DNA from the calibration phase, a standard curve was created that was a basis for conversion from imageJ INTEN values to DNA concentrations for the PCR samples. Your positive control PCR result was 90.744 μg/mL Write-in each patient ID and give both a qualitative (what the images looked like) and a quantitative description (μg/mL) of what you observed
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