BME100 s2014:T Group8 L5

BME 100 Spring 2014	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Nicole Plachecki Name: Omar Benitez Name: Colton Tucker Name: Hooyoung Kim Name: Callaway Freeland LAB 5 WRITE-UP Background Information SYBR Green Dye SYBR Dye is a photosensitive molecular dye that exhibits a green fluorescence in the dark when added to enough single-stranded DNA. Single-Drop Fluorimeter A Single Drop Fluorimeter is a device that shines blue light through a drop sample that is placed on a glass slide and detects fluorescence. Single Drop Fluorimeter. How the Fluorescence Technique Works [Instructions: In your own words] Procedure Smart Phone Camera Settings [Instructions: The type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).] Type of Smartphone: Samsung Galaxy S2 Flash: Off ISO setting: Default White Balance: Auto Exposure: Highest Saturation: Default Contrast: Default Calibration The camera phone was set up in the cradle with an angle to view the side of the drop. The fluorimeter was raised about 1 1/4 inches to accomodate for the phone height. Distance between the smart phone cradle and drop = 6.5 cm The phone was removed from this image to simplify and completely show distance setup. Distance from Fluorimeter to Cradle. [Instructions: See worksheet page 6.] Solutions Used for Calibration [Instructions: See worksheet page 6.] row 1 cell 1 row 1 cell 2 row 1 cell 3 row 1 cell 4 row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4 row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4 [Add more rows as needed] Placing Samples onto the Fluorimeter [Instructions: Step one, in your OWN words] [Instructions: Step two, in your own words] [Instructions: Step three, in your own words] [Instructions: Step etc., in your own words] Data Analysis Representative Images of Samples High DNA - using green channel Low DNA - using green channel Image J Values for All Samples [Instructions: See worksheet page 8. To save time on typing a new Wiki table from scratch, use THIS TOOL to auto-generate a Wiki table: Excel-to-Wiki Converter. Copy the headers and values from the Excel spreadsheet you made, paste them into the form field, click submit, copy the Wiki code that the tool generated, and replace TABLE GOES HERE (below) with your auto-generated code.] ' ' Area Mean Pixel Value Rawintden of the Drop Rawintden of the Background Trial 1 0 91382 71.613 6544125 18757 0.25 83776 40.875 3424354 2865 0.5 64632 64.815 4189144 373 1 72068 74.307 5355152 2461 2 74956 116.601 8739909 8280 5 81146 164.265 13329423 5734 Trial 2 0 84384 53.044 4476036 2665 0.25 60532 57.136 3458560 1701 0.5 88468 42.136 3727677 209 1 84626 85.175 7208044 106 2 87906 99.08 8709740 174 5 89884 162.199 14579066 87 Trial 3 0 97260 45.051 4381686 7168 0.25 92784 54.433 5050546 593 0.5 111748 73.74 8240277 783 1 88652 69.382 6150810 176 2 85960 100.872 8670951 175 5 93351 168.424 15722556 194 Fitting a Straight Line Intends values for each Calf Thymus DNA concentration. Intends values for each Calf THymus DNA cocnentration. PCR Results Summary Instructor's summary: You completed 8 PCR reactions in a previous lab. You used the SYBR Green I staining and imaging technique to measure the amount of amplified DNA in each PCR reaction. You used a standard curve (based on known concnetration of claf thymus DNA) to convert INTDEN values into DNA concentration. Your positive control and negative control samples are used to determine the threshold values for determining whether an unknown (patient) sample is truly positive or negative. Your positive control PCR result was ____ μg/mL Your negative control PCR result was ____ μg/mL Write-in each patient ID and give both a qualitative (what the images looked like) and a quantitative description (μg/mL) of what you observed Patient _____ : Patient _____ : Compare each patient's results to the positive control value and the negative control value. Draw a final conclusion for each patient (positive or negative) and explain why you made that conclusion. Patient _____ : Patient _____ :