BME100 s2014:T Group4 L6

BME 100 Spring 2014	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR COMPANY Name: Parker Davis Name: Daniel Munoz Name: Brian Kalen Name: Michael Spina Name: Rakan Aldrssary Company: Oxford Medical Device: PCR 3000 LAB 6 WRITE-UP Computer-Aided Design TinkerCAD TinkerCAD was an easier Solid Works. TinkerCad helpped us create our 3D and realistic image of our product. By rotating the image and adding shapes and colors you can make any 3 dimensional object. TinkerCAD has a variety of tools and devices that can be used to alter the project being made. You can put holes through the objects to make them into something like a braclet, and do things like cutting the object as well. 3D models of the PCR machine were downloaded, and then the shell of the PCR was assembled. TinkerCAD uses either hand made objects or downloaded objects in order to create projects. Our Design [Instructions: Show an image of your TinkerCAD design here] Our new design was created with the idea that the PCR machine needed to be able to hold more tubes. Therfore, to the testing bay we added additional rows and columns to the machine. Then the machine was assembled exactly the same as the normal PCR machine. The main flaw with the normal PCR machine we found it that it can only hold about 16 tubes. Adding more sockets for tubes allows for testing in a more mass amount. This can lead to drawing conclusion faster, because we will be able to test more tubes at once. This new machine will speed up the process of testing because more tubes can be tested at one time. Feature 1: Disease SNP-Specific Primers Background on the disease-associated mutation RS237025 is found in Homo sapiens. On chromosome 6:149721690. RS237025 effects the genes SUMO4 and TAB2. This SNP increases the risk of type I diabetes. The SUMO4 that is effected by rs237025 stands for small ubiquitin - like modifier 4. This SUMO4 is found in the cytoplasm and modifies IKBA which can cause type I diabetes. Primer design Disease SNP-specific Forward Primer: [Instructions: type the sequence of the forward primer] Reverse Primer: [Instructions: type the sequence of the reverse primer] How the primers work: [Instructions: explain what makes the primers disease-sequence specific. In other words, explain why the primers will amplify DNA that contains the disease-associated SNP, and will not exponentially amplify DNA that has the non-disease allele.] Feature 2: Consumables Kit [Instructions: Summarize how the consumables will be packaged in your kit. You may add a schematic image. An image is OPTIONAL and will not get bonus points, but it will make your report look awesome and easy to score.] [Instructions: IF your consumables packaging plan addresses any major weakness(es), explain how in an additional paragraph.] Feature 3: Hardware - PCR Machine & Fluorimeter [Instructions: Summarize how you will include the PCR machine and fluorimeter in your system. You may add a schematic image. An image is OPTIONAL and will not get bonus points, but it will make your report look really awesome and easy to score.] [Instructions: IF your group has decided to redesign the PCR machine and/or Fluorimeter to address any major weakness(es), explain how in an additional paragraph.] Bonus Opportunity: What Bayesian Stats Imply About The BME100 Diagnostic Approach [Instructions: This section is OPTIONAL, and will get bonus points if answered thoroughly and correctly. Here is a chance to flex some intellectual muscle. In your own words, discuss what the results for calculations 3 and 4 imply about the reliability of PCR for predicting the disease. Please do NOT type the actual numerical values here. Just refer to them as being "close to one" or "very small." The instructors will ask you to submit your actual calculations via a Blackboard quiz. We are doing so for the sake of academic integrity and to curb any temptation to cheat.]