BME100 s2014:T Group4 L5: Difference between revisions
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'''Placing Samples onto the Fluorimeter''' | '''Placing Samples onto the Fluorimeter''' | ||
# ''[Instructions: Step one, in your OWN words]''<br> | # ''[Instructions: Step one, in your OWN words]''<br> | ||
Placed 80 microliters of SYBR Green in the two middle circles. | '''Placed 80 microliters of SYBR Green in the two middle circles. ''' | ||
# ''[Instructions: Step two, in your own words]''<br> | # ''[Instructions: Step two, in your own words]''<br> | ||
Placed 80 microliters of DNA solution ontop of the Green dye. | '''Placed 80 microliters of DNA solution ontop of the Green dye.''' | ||
# ''[Instructions: Step three, in your own words]''<br> | # ''[Instructions: Step three, in your own words]''<br> | ||
Then the light was turned on from the side of the fluorimeter. | '''Then the light was turned on from the side of the fluorimeter.''' | ||
# ''[Instructions: Step four, in your own words]''<br> | # ''[Instructions: Step four, in your own words]''<br> | ||
The camera timers was started and the fluorimeter was pushed to the back of the dark box. | '''The camera timers was started and the fluorimeter was pushed to the back of the dark box.''' | ||
# ''[Instructions: Step five, in your own words]''<br> | # ''[Instructions: Step five, in your own words]''<br> | ||
The back lid of the box was shut to ensure the sample was in complete darkness. | '''The back lid of the box was shut to ensure the sample was in complete darkness.''' | ||
# ''[Instructions: Step six, in your own words]''<br> | # ''[Instructions: Step six, in your own words]''<br> | ||
Wait five seconds for the camera timer to expire, and then take the sample out and save the picture for analysis. | '''Wait five seconds for the camera timer to expire, and then take the sample out and save the picture for analysis.''' | ||
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Revision as of 10:12, 10 April 2014
BME 100 Spring 2014 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||
OUR TEAM
LAB 5 WRITE-UPBackground InformationSYBR Green Dye A photosenstive dye that rweactss with certain DNA strands. The day helps quantify how much DNA is present and therefore a sample with more DNA will appear more green. Single-Drop Fluorimeter The DNA sample is placed on a slide and then shifted into the box so the sample is in darkness.
ProcedureSmart Phone Camera Settings
[Instructions: Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.]
[Instructions: See worksheet page 6.]
[Add more rows as needed]
Placed 80 microliters of SYBR Green in the two middle circles.
Placed 80 microliters of DNA solution ontop of the Green dye.
Then the light was turned on from the side of the fluorimeter.
The camera timers was started and the fluorimeter was pushed to the back of the dark box.
The back lid of the box was shut to ensure the sample was in complete darkness.
Wait five seconds for the camera timer to expire, and then take the sample out and save the picture for analysis.
Data AnalysisRepresentative Images of Samples [Instructions: Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA] [Instructions: Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)]
Image J Values for All Samples [Instructions: See worksheet page 8. To save time on typing a new Wiki table from scratch, use THIS TOOL to auto-generate a Wiki table: Excel-to-Wiki Converter. Copy the headers and values from the Excel spreadsheet you made, paste them into the form field, click submit, copy the Wiki code that the tool generated, and replace TABLE GOES HERE (below) with your auto-generated code.]
[Instructions: Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 9]
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