BME100 s2014:T Group2 L6

BME 100 Spring 2014	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
THE CR3W Name: Bateer Song Name: Daylin Morgan Name: Ekanem-Essang Akpan Name: Hannah Churchill Name: Alexander Jones [Instructions: add the name of your team's company and/or product here] LAB 6 WRITE-UP Computer-Aided Design TinkerCAD In the beginning each part of the PCR machine was imported to Tinkercad. The files represented were 'Body: Back, Bottom, Side, Side with ports, and Top. Our Design [Instructions: A short paragraph describing your design. Why did you choose this design? How is it different from the original OpenPCR design?] Feature 1: Disease SNP-Specific Primers rs237025 is a SNP(Single nucleotide polymorphism) mainly found in Homo Sapiens. Meaning Humans are most often exhibit this variation. A nucleotide is basic unit of nucleic acid. A polymorphism is The presence of two or more phenotypes in a population because of the expression of different alleles of a given gene. rs237025 is seen on the 6:149721690 chromosome. Normally, when disease of classified, they fall under a certain clinical significance. Basically its general effect on day-to-day. That significance could be palpable, real genuine, or a noticeable effect. rs237025 did not fit any of these categories. Diseases closely linked to rs237025 are diabetes and Vogt-Koyanagi-Harada syndrome, a multi-system disease with dermatological effects. rs237025 also has the Small ubiquitin-like modifier 4(SUMO4) gene. SUMO4 is a family of genes encode small modifiers that are attached to proteins and control the target proteins’ sub-cellular localization, stability, or activity. rs237025 causes a change of the 'G' allele in the GTG genotype. An allele is Any of several forms of a gene, usually arising through mutation, that are responsible for hereditary variation, Primer design Disease SNP-specific Forward Primer: 5’ T G A A C C A C G G G G A T T G T C A G Reverse Primer: 5’ A G T T T T C T A A T T G A G A A T G C How the primers work: [ Primers are single strands of DNA that act as the beginning of DNA synthesis. Primers are set to the beginning and end of the desired DNA fragment that will be amplified. Thus this will keep it from amplifying any non-disease alleles. ] Feature 2: Consumables Kit [Instructions: Summarize how the consumables will be packaged in your kit. You may add a schematic image. An image is OPTIONAL and will not get bonus points, but it will make your report look awesome and easy to score.] [Instructions: IF your consumables packaging plan addresses any major weakness(es), explain how in an additional paragraph.] Feature 3: Hardware - PCR Machine & Fluorimeter [Instructions: Summarize how you will include the PCR machine and fluorimeter in your system. You may add a schematic image. An image is OPTIONAL and will not get bonus points, but it will make your report look really awesome and easy to score.] [Instructions: IF your group has decided to redesign the PCR machine and/or Fluorimeter to address any major weakness(es), explain how in an additional paragraph.] Bonus Opportunity: What Bayesian Stats Imply About The BME100 Diagnostic Approach [Instructions: This section is OPTIONAL, and will get bonus points if answered thoroughly and correctly. Here is a chance to flex some intellectual muscle. In your own words, discuss what the results for calculations 3 and 4 imply about the reliability of PCR for predicting the disease. Please do NOT type the actual numerical values here. Just refer to them as being "close to one" or "very small." The instructors will ask you to submit your actual calculations via a Blackboard quiz. We are doing so for the sake of academic integrity and to curb any temptation to cheat.]