BME100 s2014:T Group1 L5: Difference between revisions
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PCR Results Summary | |||
Instructor's summary: You completed 8 PCR reactions in a previous lab. You used the SYBR Green I staining and imaging technique to measure the amount of amplified DNA in each PCR reaction. You used a standard curve (based on known concentrations of calf thymus DNA) to convert INTDEN values into DNA concentration. Your positive control and negative control samples should be used as threshold values for determining whether an unknown (patient) sample is truly positive or negative. | |||
Your positive control PCR result was ____ μg/mL | |||
Your negative control PCR result was ____ μg/mL | |||
Write-in each patient ID and give both a qualitative (what the images looked like) and a quantitative description (μg/mL) of what you observed | |||
Patient 43849 : | |||
Patient 80853 : | |||
Compare each patient's results to the positive control value and the negative control value. Draw a final conclusion for each patient (positive or negative) and explain why you made that conclusion. | |||
Patient 43849 : | |||
Patient 80853 : | |||
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Revision as of 15:34, 16 April 2014
BME 100 Spring 2014 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAM
LAB 5 WRITE-UPBackground InformationSYBR Green Dye
ProcedureSmart Phone Camera Settings
Calibration
Solutions Used for Calibration
2. Get a 80 microliter measurement of the SYBR Green I and place this drop in the middle of the first two rows of the slide. The drop should look like a ball on top of the slide. 3. Get a 80 microliter measurement of the calf thymus solutions and place this solution on top of the SYBR Green I. 4. Align the slide so that the blue LED light is focused by the drop to the middle of the black fiber optic fitting on the other side of the drop. 5. Set the camera timer, then put the light box over the whole setup.
Data AnalysisRepresentative Images of Samples
Image J Values for All Samples
PCR Results Summary Instructor's summary: You completed 8 PCR reactions in a previous lab. You used the SYBR Green I staining and imaging technique to measure the amount of amplified DNA in each PCR reaction. You used a standard curve (based on known concentrations of calf thymus DNA) to convert INTDEN values into DNA concentration. Your positive control and negative control samples should be used as threshold values for determining whether an unknown (patient) sample is truly positive or negative. Your positive control PCR result was ____ μg/mL Your negative control PCR result was ____ μg/mL Write-in each patient ID and give both a qualitative (what the images looked like) and a quantitative description (μg/mL) of what you observed Patient 43849 : Patient 80853 : Compare each patient's results to the positive control value and the negative control value. Draw a final conclusion for each patient (positive or negative) and explain why you made that conclusion. Patient 43849 : Patient 80853 :
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