BME100 s2014:T Group13 L5: Difference between revisions
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'''Calibration'''<br> | '''Calibration'''<br> | ||
The camera was placed on the camera phone cradle. Plastic slides were placed under the interior black box (on which the sample slides sit), in order to line up the camera on the phone and the sample. The camera was placed 11.0 cm away from the drop in every sample in order to reduce error. | The camera was placed on the camera phone cradle. Plastic slides were placed under the interior black box (on which the sample slides sit), in order to line up the camera on the phone and the sample. The camera was placed 11.0 cm away from the drop in every sample in order to reduce error. | ||
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[[Image:Setupcalibration.PNG|400px]] | [[Image:Setupcalibration.PNG|400px]] | ||
* Distance between the smart phone cradle and drop = 11.0 cm | * Distance between the smart phone cradle and drop = 11.0 cm | ||
'''Solutions Used for Calibration' | '''Solutions Used for Calibration''' | ||
{| | {| | ||
| align="center" style="background:#f0f0f0;"|'''Initial<br>Concentration of<br>2X Calf Thymus<br>DNA Solution<br>(μg/mL)''' | | align="center" style="background:#f0f0f0;"|'''Initial<br>Concentration of<br>2X Calf Thymus<br>DNA Solution<br>(μg/mL)''' |
Revision as of 11:32, 17 April 2014
BME 100 Spring 2014 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAM
LAB 5 WRITE-UPBackground InformationSYBR Green Dye
The first component, the interior black box, acts as both a table for the slide port and a house for the blue excitation light. The plastic trays sit under this interior box and allows researchers to correctly line up the slide samples and the camera they are using. The slide port is the narrow canal in which the sample slides sit. This port allows for the all samples to be easily interchanged. The blue excitation light is a light that is shone through the samples. The next component is the camera phone cradle and the camera phone (Samsung Galaxy s4). The camera phone is used to take high quality pictures of the samples so that they can then be transferred to a computer. The cradle simply holds the camera in one place. The last component is the exterior black box. This box covers all other components and keeps all unwanted light away from the experiment.
ProcedureSmart Phone Camera Settings
Data AnalysisRepresentative Images of Samples
Instructor's summary: You completed 8 PCR reactions in a previous lab. You used the SYBR Green I staining and imaging technique to measure the amount of amplified DNA in each PCR reaction. You used a standard curve (based on known concentration of calf thymus DNA) to convert INTDEN values into DNA concentration. Your positive control and negative control samples are used to determine the threshold values for determining whether an unknown (patient) sample is truly positive or negative.
Compare each patient's results to the positive control value and the negative control value. Draw a final conclusion for each patient (positive or negative) and explain why you made that conclusion.
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