BME100 s2014:T Group13 L5: Difference between revisions
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The Fluorimeter allows for the quantitative evaluation of samples that underwent a Polymerase Chain Reaction (PCR). It is made up of an interior black box, or table, plastic trays, a slide port, a blue excitation light, a camera phone cradle, a Samsung galaxy s4 camera phone, and an exterior black box that covers all other components. The first component, the interior black box, basically acts as both a table for the slide port and a house for the blue excitation light. The plastic trays sit under this interior box and allows researchers to correctly line up the slide samples and the camera they are using. The slide port is the narrow canal in which the sample slides sit. This port allows for the all samples to be easily interchanged but still remain in the same place on the table (in line with the camera). The blue excitation light is a blue light that is shone through the selected samples. When the light shines through samples with DNA, the sample will absorb the blue light and reflect a green light. The next component is the camera phone cradle and the camera phone (Samsung Galaxy s4). The camera phone is used to take high quality pictures of the samples so that they can then be transferred to a computer and quantitatively evaluated. The cradle simply holds the camera in one place (in line with the sample slides and the blue light). The last component is the exterior black box. This box covers all other components and keeps all unwanted light away from the experiment. This allows for the camera to detect all the green light that is being reflected from the sample. | The Fluorimeter allows for the quantitative evaluation of samples that underwent a Polymerase Chain Reaction (PCR). It is made up of an interior black box, or table, plastic trays, a slide port, a blue excitation light, a camera phone cradle, a Samsung galaxy s4 camera phone, and an exterior black box that covers all other components. The first component, the interior black box, basically acts as both a table for the slide port and a house for the blue excitation light. The plastic trays sit under this interior box and allows researchers to correctly line up the slide samples and the camera they are using. The slide port is the narrow canal in which the sample slides sit. This port allows for the all samples to be easily interchanged but still remain in the same place on the table (in line with the camera). The blue excitation light is a blue light that is shone through the selected samples. When the light shines through samples with DNA, the sample will absorb the blue light and reflect a green light. The next component is the camera phone cradle and the camera phone (Samsung Galaxy s4). The camera phone is used to take high quality pictures of the samples so that they can then be transferred to a computer and quantitatively evaluated. The cradle simply holds the camera in one place (in line with the sample slides and the blue light). The last component is the exterior black box. This box covers all other components and keeps all unwanted light away from the experiment. This allows for the camera to detect all the green light that is being reflected from the sample. | ||
[[Image:Fluorimter.PNG| | [[Image:Fluorimter.PNG|390px]] | ||
Revision as of 11:04, 17 April 2014
BME 100 Spring 2014 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||||
OUR TEAM
LAB 5 WRITE-UPBackground InformationSYBR Green Dye
ProcedureSmart Phone Camera Settings
Calibration
[Instructions: See worksheet page 6.]
[Add more rows as needed]
Data AnalysisRepresentative Images of Samples
[Instructions: See worksheet page 8. To save time on typing a new Wiki table from scratch, use THIS TOOL to auto-generate a Wiki table: Excel-to-Wiki Converter. Copy the headers and values from the Excel spreadsheet you made, paste them into the form field, click submit, copy the Wiki code that the tool generated, and replace TABLE GOES HERE (below) with your auto-generated code.]
Instructor's summary: You completed 8 PCR reactions in a previous lab. You used the SYBR Green I staining and imaging technique to measure the amount of amplified DNA in each PCR reaction. You used a standard curve (based on known concnetration of claf thymus DNA) to convert INTDEN values into DNA concentration. Your positive control and negative control samples are used to determine the threshold values for determining whether an unknown (patient) sample is truly positive or negative.
Compare each patient's results to the positive control value and the negative control value. Draw a final conclusion for each patient (positive or negative) and explain why you made that conclusion.
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