BME100 s2014:T Group10 L5

BME 100 Spring 2014	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Sean-Christopher Bradbury Thursday Group 10 Member Suyen Go Thursday Group 10 Member Nathaniel Kirkpatrick Thursday Group 10 Member Maya Robinson Thursday Group 10 Member Lissette Valle Thursday Group 10 Member LAB 5 WRITE-UP Background Information SYBR Green Dye [Instructions: A short description of SYBR Green Dye device.] SYBR Green Dye is a dye that stains nucleic acids by binding to DNA or RNA. The dye is used to visualize DNA through staining agarose gels, biochip applications, fluorescence imaging techniques, real-time PCR, and more. SYBR dye fluoresces when combined with dsDNA (Zipper Hubert Brunner 1). Single-Drop Fluorimeter [Instructions: A description of the single-drop fluorimeter device. Add a PHOTO for bonus points] A fluorimeter is a device used to measure and identify fluorescence in a medium. Fluorimeters measure certain wavelengths of light and have two light detectors, one of which detects absorbency while the other detects fluorescence emission (So Dong 1). How the Fluorescence Technique Works In this experiment, the concentration of DNA was determined by measuring the amount of fluorescence in a medium which had been dyed with SYBR Green Dye. A single-drop fluorimeter was used to measure the fluorescence and an image was taken to be analyzed with ImageJ software to determine the concentration of DNA. Citations: Zipper, Hubert, Herwig Brunner, Jurgen Bernhagen, and Frank Vitzthum. "Investigations on DNA Intercalation and Surface Binding by SYBR Green I, Its Structure Determination and Methodological Implications." Ncbi.nih.gov. US National Library of Medicine, 12 July 2004. Web. 3 Apr. 2014. So, Peter TC, and Chen Y. Dong. "Fluorescence Spectrophotometry." Scribd. Macmillan Publishers Ltd, Nature Publishing Group, 16 Jan. 2009. Web. 03 Apr. 2014. Procedure Smart Phone Camera Settings [Instructions: The type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).] Type of Smartphone: Apple iPhone5s Flash: no ISO setting: White Balance: Exposure: Saturation: Contrast: Calibration [Instructions: Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.] Distance between the smart phone cradle and drop = 8cm [Instructions: See worksheet page 6.] Solutions Used for Calibration [Instructions: See worksheet page 6.] row 1 cell 1 row 1 cell 2 row 1 cell 3 row 1 cell 4 row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4 row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4 [Add more rows as needed] Placing Samples onto the Fluorimeter [Instructions: Step one, in your OWN words] [Instructions: Step two, in your own words] [Instructions: Step three, in your own words] [Instructions: Step etc., in your own words] Data Analysis Representative Images of Samples [Instructions: Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA] [Instructions: Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)] 1R1 traced with oval tool. Positive Control 1. Positive Control 2. Positive Control 3. 1R1 1. 1R2 2. 1R2 3. Image J Values for All Samples [Instructions: See worksheet page 8. To save time on typing a new Wiki table from scratch, use THIS TOOL to auto-generate a Wiki table: Excel-to-Wiki Converter. Copy the headers and values from the Excel spreadsheet you made, paste them into the form field, click submit, copy the Wiki code that the tool generated, and replace TABLE GOES HERE (below) with your auto-generated code.] TABLE GOES HERE Fitting a Straight Line [Instructions: Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 9]