BME100 f2016:Group4 W1030AM L5

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OUR TEAM Matt Morton Mahina Wing Jaeger Moore Trevor Wood LAB 5 WRITE-UP PCR Reaction Report Pipetting the samples ton set of the reaction was fairly simple and straightforeward. If we conducted this lab without watching the pre-lab videos of how to properly use a micropipette this lab would have had much more error occur within our PCR reaction and we would have struggled with taking photos of the cyber-green substance mixed with our samples and the different concentrations of DNA samples that we also tested. The difference between the first stop versus the second stop on the pipette is also clear with the first stop being used to accurately collect the exact amount of fluid we needed and using the second stop to then ensure that all collected fluid is expelled out of the micro-pipette tip. Using those stops integrated on the pipette ensured that all of our final reactions received the same amount of fluid, reducing any possible errors due to concentration in our data. However there was lequid left in all of our DNA tubes after we conducted the experiment using cyber-green but this is expected as we used the fluid that was in those tubes to dilute the DNA from our PCR reactions. We also did not have to change our labeling scheme because we used simple labels that were similar to the ones given on the instructions for the lab to prevent confusion within out group. As our skills improved using the pippetes within this lab, it made analyzing the effects of the PCR reaction much easier to understand as we minimized error in our data to interpret. Fluorimeter Procedure Imaging set-up First we placed the call phone with a camera on (Samsung Galaxy s7 edge) it into the given stand that came in the light box. To secure the cell phone into the stand that it is in we then also placed a pencil to that the cellphone was standing almost perpendicular to the desk that it was placed on. After setting up the cell phone in its stand we then went about setting up the Florimiter so that the viewing platform of it was within almost level with the camera. To do this the florimeter had to be placed onto a few of the generic trays until an optimal height to where the camera is still a few centimeters above the viewing platform of the florometer. Placing Samples onto the Fluorimeter [Instructions: Step one, in your own words] [Instructions: Step two, in your own words] [Instructions: Step three, in your own words] [Instructions: Step etc., in your own words] Data Collection and Analysis Images of High, Low, and Zero Calf Thymus DNA Calibrator Mean Values TABLE GOES HERE Calibration curves Images of Our PCR Negative and Positive Controls PCR Results: PCR concentrations solved TABLE GOES HERE PCR Results: Summary Our positive control PCR result was ____ μg/mL Our negative control PCR result was ____ μg/mL Observed results Patient _____ : Patient _____ : Conclusions Patient _____ : Patient _____ :