BME100 f2016:Group1 W8AM L5

BME 100 Fall 2016	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Audriana Sedmak Name: Benjamin Robles Name: Emma Rodriguez Name: Mitchell Miranda Name: Spencer Brimley LAB 5 WRITE-UP PCR Reaction Report To start the experiment samples of DNA from two separate patients was provided, as well as a sample that was known as positive for PCR gene and one that was known to be negative for the PCR gene, and PRC reaction mix. A micropipette was used to transfer 50 uL of each sample of DNA from the patients as well as the positive and negative DNA samples into knew PCR reaction tubes. Each sample was put into a different tube, and a new pipette tip was used for each sample. 50 uL of the PCR reaction mix was then placed into each different tube, with a new pipette tip was used each time again to keep the samples pure.Visually it appeared as though all of the tubes had the same volume after the DNA samples and the PCR reaction mix had been added. The new reaction mixes were placed into the PCR machine which ran until the reaction was run to completion. When using a micropipette it is important to change the tip every time you work with a new sample and to set the desired volume on the pipette. The first stop verses the second stop of the pipette insures that the solution is not let out too quickly and also ensures that all of the solution is gotten out of the pipette tip. Fluorimeter Procedure Imaging set-up Set up the light box so the front is open. Set up the fluorimeter by placing it on a clean, flat surface. Position the camera so that it is able to "see" the top of the sample slides and view the drops horizontally. Insert slide facing smooth-side down. Placing Samples onto the Fluorimeter Place 80 µl of SYBR green between the first two rows of the slide. Add 80 µl of the DNA sample to the SYBR green. Using a timer, take a picture of the sample on the fluorimeter inside the light box. Remove the 160 µl sample and move the slide to the next row in preparation for the next sample. Repeat steps 3 thru 6 for all the samples. Data Collection and Analysis Images of High, Low, and Zero Calf Thymus DNA Calibrator Mean Values TABLE GOES HERE Calibration curves Images of Our PCR Negative and Positive Controls PCR Results: PCR concentrations solved TABLE GOES HERE PCR Results: Summary Our positive control PCR result was ____ μg/mL Our negative control PCR result was ____ μg/mL Observed results Patient _____ : Patient _____ : Conclusions Patient _____ : Patient _____ :