BME100 f2016:Group1 W1030AM L5: Difference between revisions
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| '''Initial Concentration of 2X Calf Thymus DNA solution (micrograms/mL)''' || '''Final DNA concentration in SYBR Green I solution (μg/mL)''' || '''Sample Number''' | | '''Initial Concentration of 2X Calf Thymus DNA solution (micrograms/mL)''' || '''Final DNA concentration in SYBR Green I solution (μg/mL)''' || '''Sample Number''' || '''RAWINTDEN DROP-BACKGROUND''' || '''MEAN''' || '''Standard Deviation''' | ||
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| G1 + || Positive control || none | | G1 + || Positive control || none |
Revision as of 12:27, 26 October 2016
BME 100 Fall 2016 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||
OUR TEAM
LAB 5 WRITE-UPPCR Reaction Reporthere's a task guys!! Fluorimeter ProcedureImaging set-up First, the fluorimeter was set up. A hydrophobic slide was placed onto the device with its smooth side down. The blue LED light was turned on to lighten the first two rows of the slide. A cradle with a smartphone on it was set to take pictures of the slide. The distance between the camera of the smartphone and the first two rows of the slide was adjusted in a way that the camera was focused at that part of the slide and as close to it as possible. It was also important to higher the fluorimeter (in our experiment, we used plastic trays to do so) so that the camera would take pictures of the drops on the slide sideways.
Placing Samples onto the Fluorimeter
Data Collection and AnalysisImages of High, Low, and Zero Calf Thymus DNA
Images of Our PCR Negative and Positive Controls
PCR Results: PCR concentrations solved TABLE GOES HERE
PCR Results: Summary
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