BME100 f2016:Group1 W1030AM L5: Difference between revisions

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'''Imaging set-up'''<br>
'''Imaging set-up'''<br>
<!-- INSTRUCTIONS: In the space below, describe in detail how your team set up your device to capture images from the fluorimeter. -->
<!-- INSTRUCTIONS: In the space below, describe in detail how your team set up your device to capture images from the fluorimeter. -->
 
First, the fluorimeter was set up. A hydrophobic slide was placed onto the device with its smooth side down. The blue LED light was turned on to lighten the first two rows of the slide. A cradle with a smartphone on it was set to take pictures of the slide. The distance between the camera of the smartphone and the first two rows of the slide was adjusted in a way that the camera was focused at that part of the slide and as close to it as possible. It was also important to higher the fluorimeter (in our experiment, we used plastic trays to do so) so that the camera would take pictures of the drops on the slide sideways.




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<!-- INSTRUCTIONS: In the space below, in your own words write the steps you performed to place samples onto the fluorimeter -->
<!-- INSTRUCTIONS: In the space below, in your own words write the steps you performed to place samples onto the fluorimeter -->


# ''[Instructions: Step one, in your own words]''
# ''[Step 1: ]''
# ''[Instructions: Step two, in your own words]''
# ''[Instructions: Step two, in your own words]''
# ''[Instructions: Step three, in your own words]''
# ''[Instructions: Step three, in your own words]''

Revision as of 12:04, 26 October 2016

BME 100 Fall 2016 Home
People
Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
Course Logistics For Instructors
Photos
Wiki Editing Help

OUR TEAM

Carlye A. Frisch
Kendra M. Gibble
Anna Rothweil
Gabrielle F. Wipper
Nicholas C. Whitley


LAB 5 WRITE-UP

PCR Reaction Report

Fluorimeter Procedure

Imaging set-up

First, the fluorimeter was set up. A hydrophobic slide was placed onto the device with its smooth side down. The blue LED light was turned on to lighten the first two rows of the slide. A cradle with a smartphone on it was set to take pictures of the slide. The distance between the camera of the smartphone and the first two rows of the slide was adjusted in a way that the camera was focused at that part of the slide and as close to it as possible. It was also important to higher the fluorimeter (in our experiment, we used plastic trays to do so) so that the camera would take pictures of the drops on the slide sideways.


Placing Samples onto the Fluorimeter

  1. [Step 1: ]
  2. [Instructions: Step two, in your own words]
  3. [Instructions: Step three, in your own words]
  4. [Instructions: Step etc., in your own words]


Data Collection and Analysis

Images of High, Low, and Zero Calf Thymus DNA


Calibrator Mean Values


TABLE GOES HERE


Calibration curves


Images of Our PCR Negative and Positive Controls


PCR Results: PCR concentrations solved

TABLE GOES HERE


PCR Results: Summary

  • Our positive control PCR result was ____ μg/mL
  • Our negative control PCR result was ____ μg/mL


Observed results

  • Patient _____ :
  • Patient _____ :


Conclusions

  • Patient _____ :
  • Patient _____ :