BME100 f2016:Group12 W1030AM L5

BME 100 Fall 2016	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: student Name: student Name: student Name: student Name: student Name: student LAB 5 WRITE-UP PCR Reaction Report The pre-lab reading was very helpful because it explained in depth how to pipette. We were confident when we began pipetting because of the simulations that we did before class. We all understood the difference between the first and second stop. Before we began using the actual solutions, we practiced with the micropipettor to find the first and seconds stop. There was no noticeable difference in the amount of liquid in the final reactions. There was no liquid left in the tubes that contained the DNA samples and the PCR reaction mix. We did change our labeling scheme a little. We kept the G12 the same on all the tubes but then we labeled the tubes with patient ID 37106 with the labels 3-1, 3-2, and 3-3. We labeled the tubes with patient ID 54597 with the labels 5-1, 5-2, and 5-3. Fluorimeter Procedure Imaging set-up In the light box, the flourimeter was placed on top of two boxes to prop it up to the height of the camera. A smartphone camera was placed 8 cm away from the light in the flourimeter in a smartphone cradle. The flash was disabled on the camera in order to get a clear picture. The timer was set on the camera for 10 seconds so the light box could be closed and the picture would be taken in darkness. The camera was focused so the picture would be clear. See picture below for complete set up. Placing Samples onto the Fluorimeter Place slide in flourimeter with rough side up. Using a micropipettor place 80 mircoliter of SYBR GREEN 1 on the slide between the first two rows. The drop should be round, balled shaped. Add 80 microliters of a calf thymus solution to the SYBR GREEN 1 drop. Move the slide so the light is in the middle of the drop. Take a picture using the timer on the smartphone camera while the light box is covering the complete set up so as much light it removed as possible. Take three focused pictures of the drop. Remove the box being careful to keep the set up in place. Use the micropipettor to remove the drop and discard the waste. Repeat these sets for each concentration of the calf thymus solution. Data Collection and Analysis Images of High, Low, and Zero Calf Thymus DNA Calibrator Mean Values TABLE GOES HERE Calibration curves Images of Our PCR Negative and Positive Controls PCR Results: PCR concentrations solved TABLE GOES HERE PCR Results: Summary Our positive control PCR result was ____ μg/mL Our negative control PCR result was ____ μg/mL Observed results Patient _____ : Patient _____ : Conclusions Patient _____ : Patient _____ :