BME100 f2016:Group10 W1030AM L5
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OUR TEAM
LAB 5 WRITE-UPPCR Reaction ReportThe pre-lab reading was very interactive and informative. For those who have never used a micropipette, the pre-lab information was beneficial. The first stop on the pipette is used to collect the sample that is being transferred and the second stop is used to release the sample liquid. The final reaction will have the same amount of liquid. There was no liquids left in the tubes of the DNA samples and PCR reaction mix. Fluorimeter ProcedureImaging set-up A member in our group provided their iPhone 6s camera in order to take images while the fluorimeter measures the fluorescence of the DNA. This is the transmittance of the wavelength given off by the DNA that is excited. The wavelength of light is exciting the DNA and if it fluoresces then there is double-stranded DNA present. Taking the iPhone 6s, the camera's flash was inactived in order to get a reasonable image of the calibration with the DNA concentration. The iPhone 6s was placed on to a metal card holder and the metal card holder was put on top of a plastic stand at a distance of 4 centimeters. The iPhone 6s was leaning on an angle due to the metal card holder and we wanted to have the camera perpendicular to the fluorimeter. In order to do this, we placed a folded piece of paper behind the iPhone 6s to prop it up.
Data Collection and AnalysisImages of High, Low, and Zero Calf Thymus DNA
Images of Our PCR Negative and Positive Controls
PCR Results: PCR concentrations solved TABLE GOES HERE
PCR Results: Summary
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