BME100 f2015:Group8 1030amL1

BME 100 Fall 2015	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Sydney Connor Name: Tajinder Virdee Name: Michael Dagher Name: Olivia Gonzalez Name: Alaina Jenish Name: Angela Hemesath LAB 1 WRITE-UP Independent and Dependent Variables The independent variable in this experiment will be the amount in milligrams of lipopolysaccharide given to each of the five groups. Lipopolysaccharide is the independent variable because it is the drug that is being varied to measure the amount of inflammation in this experiment The dependent variable in this experiment will be the levels of the protein Inflammotin in the blood stream of the test subjects. The level of inflammation is being measured and therefore depends on the amount of the Lipopolysaccharide being taken. Experimental Design A double-blind experiment is conducted to measure the protein Inflammation level of each subject before and after a dose of lipopolysaccharide is administered. Each group is administered a different level of lipopolysaccharide: 0 mg, 2.5 mg, 5.0 mg, 7.5 mg, and 10 mg. These increasing levels of the drug allow a determination of the lowest range at which the drug functions. A total sample of 100 elders between the ages of 65-85, randomly selected from the Social Security data base, are split into 5 groups. This age range allows a wide variety of subjects and includes subjects who are elderly but are healthy and strong enough to withstand the drug. Each of the 5 groups contains 20 people, consisting of 5 elders from the ages of 65-70, 5 of 70-75, 5 of 75-80, and 5 of 80-85. Specific age ranges are implemented in order to decrease biases in the sample (for example, if one group were all 80+). The elders of each age group are then randomly placed into groups. Two groups of 20 elders act as controls; one group of 20 is given a placebo which contains no drug, and another group is given the highest dosage of the drug at 10 mg, to validate that the drug will have taken effect and to compare the two extremes of the drug. Of the three remaining groups, one is given a dosage of 2.5 mg, the second 5.0 mg, and the third 7.5 mg. Each member of all the groups is tested for inflammation levels before taking the drug. Then, after taking the drug, each member of each group is tested four times: 30 minutes, 60 minutes, 90 minutes and 120 minutes after taking the drug. The subjects are tested every day for a week and each group will take the same dosage as they did before, in order to repeat the test and minimize error. During each test, neither the experimenter nor the subject will know what dosage they are administering/given, so that there is no placebo effect or observer-expectancy effect. This reduces bias and error in the experiment. Groups Age Number of subjects per group Subject Selection Sources of Error and Bias Sources of Error in the experiment may include other health problems that each individual may have, such as allergies or certain medical conditions that may impact the blood results. Another source of error in this experiment may include other medicines or treatments that each of the subjects is currently taking that could potentially impact the protein levels in the test subjects' blood system. Since the sample population only includes people of the age 65 to 85 who are registered with the Social Security Administration in the United States, another source of error may be created by not including people who are not registered with the Social Security Administration. Therefore this experiment does not measure subjects from outside the United States, which may create different results had this experiment been conducted on the global scale.