BME100 f2015:Group1 1030amL1

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OUR TEAM

Name: Matt Chrest
Name: Nichole Torgerson
Name: Lindsey O'Brien
Name: Jacob Aperi
Name: Michael "Otis" Clyne
Name: Alarmel Sira

LAB 1 WRITE-UP

Independent and Dependent Variables

Independent Variable: Dosage of lipopolysaccharide administered
Dependent Variable: Increase or decrease of Inflammotin concentration in the blood

Experimental Design

Our Experiment will consist of 4 different groups, divided up by dosage size. Since it is known that 10mg of the drug produces inflammation, our dosages are marked down in increments of 2mg, in order to find the minimum dosage needed for inflammation. Each group's levels of the inflammatory protein will be measured before taking the pill in order to get a baseline for our subjects. Subjects' Inflammotin concentration will be measured at two hour increments for the following six hours to see how the subjects' Inflammotin levels are affected by Lipopolysaccharide. The subjects' Inflammotin levels will be measured by blood drawn at the 2 hour increments.


Groups

1. 2mg of lipopolysaccharide

2. 4mg of lipopolysaccharide

3. 6mg of lipopolysaccharide

4. 8mg of lipopolysaccharide

Age
65-85 years


Number of subjects per group
25





Subject Selection

100 people between the ages 65-85. Randomly selected individuals for randomized ages.






Sources of Error and Bias

Possible sources of error are factors that could interfere with Inflammotin concentration in the blood. An example is general health factors like weight and previous illnesses. A second example is if the subject is taking anti-inflammatory drugs.

Randomization when providing the dosages of the pills will help eliminate the outliers and reduce the skew of data.