BME100 f2015:Group16 1030amL4: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> BME 100 Fall 2015</span>
|style="background-color: #F2F2F2" | [[BME100_f2015 | <font face="trebuchet ms" style="color: #808080"> '''Home''' </font>]]<br>[[BME100_f2015:People | <font face="trebuchet ms" style="color: #808080"> '''People''' </font>]]<br>[[BME100_f2015:Projects1 | <font face="trebuchet ms" style="color: #808080"> '''Lab Write-Up 1''' </font>]] | [[BME100_f2015:Projects2 | <font face="trebuchet ms" style="color: #808080"> '''Lab Write-Up 2''' </font>]] | [[BME100_f2015:Projects3 | <font face="trebuchet ms" style="color: #808080"> '''Lab Write-Up 3''' </font>]]<br>[[BME100_f2015:Projects4 | <font face="trebuchet ms" style="color: #808080"> '''Lab Write-Up 4''' </font>]] | [[BME100_f2015:Projects5 | <font face="trebuchet ms" style="color: #808080"> '''Lab Write-Up 5''' </font>]] | [[BME100_f2015:Projects6 | <font face="trebuchet ms" style="color: #808080"> '''Lab Write-Up 6''' </font>]]<br>[[BME100_f2015:Logistics | <font face="trebuchet ms" style="color: #808080"> ''' Course Logistics For Instructors''' </font>]] <br>[[BME100_f2015:Photos | <font face="trebuchet ms" style="color: #808080"> '''Photos''' </font>]] <br>[[BME100_f2015:WikiHelp | <font face="trebuchet ms" style="color: #808080"> '''Wiki Editing Help''' </font>]]
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=OUR TEAM=
{| style="wikitable" width="700px"
|- valign="top"
| [[Image:BME103student.jpg|100px|thumb|Name: student<br>Role(s)]]
| [[Image:BME103student.jpg|100px|thumb|Name: student<br>Role(s)]]
| [[Image:BME103student.jpg|100px|thumb|Name: student<br>Role(s)]]
| [[Image:BME103student.jpg|100px|thumb|Name: student<br>Role(s)]]
| [[Image:BME103student.jpg|100px|thumb|Name: student<br>Role(s)]]
| [[Image:BME103student.jpg|100px|thumb|Name: student<br>Role(s)]]
|}
=LAB 4 WRITE-UP=
==Protocol==
'''Materials'''
<!-- Record all of the materials you will need for PCR -->
* Item 1
* Item 2
* etc.
'''PCR Reaction Sample List'''<br>
<!-- Fill in ALL of the blank cells. Replace the # symbols with your group’s number. For help with tables, see Wiki editing help in the main menu -->
{| {{table}}
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| '''Tube Label''' || '''PCR Reaction Sample''' || '''Patient ID'''
|-
| G# + || Positive control || none
|-
| G# - || Negative control || none
|-
| G# 1-1 || Patient 1, replicate 1 ||
|-
| G# 1-2 || Patient 1, replicate 2 ||
|-
| G# 1-3 || Patient 1, replicate 3 ||
|-
| G# 2-1 || Patient 2, replicate 1 ||
|-
| G# 2-2 || Patient 2, replicate 2 ||
|-
| G# 2-3 || Patient 2, replicate 3 ||
|}
'''DNA Sample Set-up Procedure'''
<!-- In your own words, write an easy-to-comprehend list of the steps your group took to set up the PCR reaction in the PCR reaction tubes. End with placing the tubes in to the thermal cycler. Do not copy-paste the instructions from the Workbook. That will be considered plagiarism. -->
# Step 1
# Step 2
# Step 3...
'''OpenPCR program'''
<!-- Include a description of the thermal cycling program below. You can use text, a screen capture, a camera snapshot of the computer screen, or a digital drawing (e.g., using shapes and text boxes in Microsoft Powerpoint) -->
<br><br>
==Research and Development==
'''PCR - The Underlying Technology'''<br>
<!-- Add a write-up, essay-style, organized into paragraphs with descriptive headers, based on the questions and answers from the Research and Development exercise. BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the PCR video/ tutorial might be useful. Be sure to '''credit the sources''' if you borrow images. You are not allowed to use images from current or past BME 100 students' reports on OpenWetWare. -->
<br><br>
==SNP Information & Primer Design==
'''Background: About the Disease SNP'''
<!-- INSTRUCTIONS: This content is from PCR Lab B. Write a summary, at least five sentences long, about the disease SNP in your own words. -->
'''Primer Design and Testing'''
<!-- INSTRUCTIONS: Write a short summary of the results of your primer test. Underneath your summary, include a screen capture of the results web page. You may crop the image so that it only includes the relevant information. -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
|}

Revision as of 11:12, 14 October 2015

BME 100 Fall 2015 Home
People
Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
Course Logistics For Instructors
Photos
Wiki Editing Help


OUR TEAM

Name: student
Role(s)
Name: student
Role(s)
Name: student
Role(s)
Name: student
Role(s)
Name: student
Role(s)
Name: student
Role(s)

LAB 4 WRITE-UP

Protocol

Materials

  • Item 1
  • Item 2
  • etc.


PCR Reaction Sample List

Tube Label PCR Reaction Sample Patient ID
G# + Positive control none
G# - Negative control none
G# 1-1 Patient 1, replicate 1
G# 1-2 Patient 1, replicate 2
G# 1-3 Patient 1, replicate 3
G# 2-1 Patient 2, replicate 1
G# 2-2 Patient 2, replicate 2
G# 2-3 Patient 2, replicate 3


DNA Sample Set-up Procedure

  1. Step 1
  2. Step 2
  3. Step 3...


OpenPCR program






Research and Development

PCR - The Underlying Technology






SNP Information & Primer Design

Background: About the Disease SNP


Primer Design and Testing




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