BME100 f2015:Group13 8amL1

BME 100 Fall 2015	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Shivanshi Shukla Name: Ksenia Poujlivaia Name: Alexander DaSilva Name: Parker Storry Name: Luis Morales Name: Derek Scott LAB 1 WRITE-UP Independent and Dependent Variables Independent Variable: Our goal is to measure the minimum amount of lipopolysaccharide needed to induce the presence of inflammatin, thus, the amount, in milligrams(mg), of lipopolysaccharide given to the test subject is our independent variable. Dependent Variable: Presence and amount of inflammotin in test subject after being given the assigned dosage of lipopolysaccharide. Experimental Design Groups 6 groups total: Each of the six groups will be given dosages of the agent lipopolysaccharide, beginning at 0mg for the control and ending with 10mg administered to Group 6, increased between groups by 2mg: Group 1 (Control Group) will be given 0mg of lipopolysaccharide; Group 2 will be given a 2mg dosage; Group 3 will be given a 4mg dosage Group 4 will be given a 6mg dosage Group 5 will be given a 8mg dosage Group 6 will be given a 10mg dosage Note: Prior work has shown that a 10mg dose of lipopolysaccharide will induce the presence of Inflammotin. Age Test subject will be selected from within the ages of 60-80 years old. Number of subjects per group Twenty (20) subjects will be assigned to each experimental group. five (5) people within each age range, as described below, will be in each group. five (5) people within the ages of 60-64; five (5) people from within the ages of 65-70; five (5) people within the ages of 70-74; and, five (5) people within the ages of 75-80. Subject Selection Requirements: Within the ages of 60-80 years old. Only subjects without the protein Inflammotin in their system naturally will be selected for the test phase to prevent prior levels to skew results. Subjects will have to provide health records for review and only subjects with minimal health problems will be accepted. Current medications and health issues will be evaluated as to assure that being given lipopolysaccharide will not negatively affect their health or the tests. To combat randomization and bias people will be from various ethnic backgrounds and gender as to not bias one ethnicity or gender. Each group will have one subject of each age ranging from 60-80 to reiterate randomization and prevent bias against a certain age. Sources of Error and Bias Sources of error: One source of error could come from the fact that people are aware of the effects of the lipopolysaccharide. Another error source could come from improper collecting of blood samples leading to skewed results. A third error source could come from outside influences such as diet, stress, and etc. Controls for the sources of error: In order to prevent people's perceptions from skewing data, the experiment will be double blind so researchers and subjects do not react to knowledge of the dosage being administered. To combat the improper collecting of data, multiple samples will be taken and compared to previous samples of the subject to eliminate any outliers in the information collected. To control the changes made from outside influences, the awareness that things other than the drug could change Inflammotin levels allows researchers to give rationale for any levels that do not follow the expected results in the hypothesis.