BME100 f2015:Group11 8amL5: Difference between revisions
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''' | '''Camera set-up'''<br> | ||
<!-- INSTRUCTIONS: In the space below, briefly describe how to set up your camera in front of the fluorimeter | <!-- INSTRUCTIONS: In the space below, briefly describe how to set up your camera in front of the fluorimeter. --> | ||
<!-- INSTRUCTIONS: Type the distance between your phone cradle and the drop after the equal sign. --> | <!-- INSTRUCTIONS: Type the distance between your phone cradle and the drop after the equal sign. --> | ||
* Distance between the smart phone cradle and drop = | * Distance between the smart phone cradle and drop = | ||
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<!-- Note: Be sure to delete the instruction text in brackets: ''[ ]'' --> | <!-- Note: Be sure to delete the instruction text in brackets: ''[ ]'' --> | ||
==Data Analysis== | ==Data Collection and Analysis== | ||
'''Images of High, Low, and Zero Calf Thymus DNA''' | |||
<!-- INSTRUCTIONS: Show ONE image where you drew a circle around the droplet in ImageJ for any image for the (1) 5 μg/mL sample (2) 0.5 μg/mL sample and (3) zero DNA. Please crop your images so that only the drop and a small empty rectangular region around the drop are included. Lots of empty space is a waste of space. --> | |||
'''Calibrator Mean Values''' | |||
<!-- INSTRUCTIONS: Show all values from Excel Table 2 from Section 3. '''To save time on typing a new Wiki table from scratch''', use THIS TOOL to auto-generate a Wiki table: http://excel2wiki.net/wikipedia.php. Copy the headers and values from the Excel spreadsheet you made, paste them into the form field, click submit, copy the Wiki code that the tool generated, and replace TABLE GOES HERE (below) with your auto-generated code. --> | |||
TABLE GOES HERE | |||
'''Calibration curves'''<br> | |||
<!-- INSTRUCTIONS: Place images of your Excel plots (2 total) here. --> | |||
'''Images of Our PCR Negative and Positive Controls''' | |||
<!-- INSTRUCTIONS: Show ONE image where you drew a circle around the droplet in ImageJ for any image for the (1) Negative control PCR sample AND (2) the Positive control PCR sample. --> | |||
'''PCR Results: PCR concentrations solved''' | |||
<!-- INSTRUCTIONS: Show all values from Excel Table 5 from Section 5. '''To save time on typing a new Wiki table from scratch''', use THIS TOOL to auto-generate a Wiki table: http://excel2wiki.net/wikipedia.php. Copy the headers and values from the Excel spreadsheet you made, paste them into the form field, click submit, copy the Wiki code that the tool generated, and replace TABLE GOES HERE (below) with your auto-generated code. --> | |||
TABLE GOES HERE | TABLE GOES HERE | ||
'''PCR Results Summary''' | '''PCR Results: Summary''' | ||
<!-- INSTRUCTIONS: You completed 8 PCR reactions and used the SYBR Green I staining and imaging technique to measure the amount of amplified DNA in each PCR reaction. You used a standard curve (based on known concentrations of calf thymus DNA) to convert INTDEN values into DNA concentration. Your positive control and negative control samples should be used as '''threshold''' values for determining whether an unknown (patient) sample is truly positive or negative. Replace the underscore with your | <!-- INSTRUCTIONS: You completed 8 PCR reactions and used the SYBR Green I staining and imaging technique to measure the amount of amplified DNA in each PCR reaction. You used a standard curve (based on known concentrations of calf thymus DNA) to convert INTDEN values into DNA concentration. Your positive control and negative control samples should be used as '''threshold''' values for determining whether an unknown (patient) sample is truly positive or negative. Replace the underscore with your calculated initial concentration values.--> | ||
* Our positive control PCR result was ____ μg/mL | * Our positive control PCR result was ____ μg/mL | ||
* Our negative control PCR result was ____ μg/mL | * Our negative control PCR result was ____ μg/mL | ||
<u>Observed results</u> | <u>Observed results</u> | ||
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* Patient _____ : | * Patient _____ : | ||
* Patient _____ : | * Patient _____ : | ||
<u>Conclusions</u> | <u>Conclusions</u> |
Revision as of 10:05, 28 October 2015
BME 100 Fall 2015 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | ||||||
OUR TEAM
LAB 5 WRITE-UPPCR Reaction ReportBefore attending lab, we read the pre-lab reading and completed the quiz. THis prepareed us for pipetting the DNA samples and the PCR reaction mix. Each DNA sample, as well as the positive and negative control were added to each labelled PCR reaction tube. We labelled the tubes on the front side using a sharpie: P (positive), N (negative), 1 (patient 1), 2 (patient 2). We pipetted, drawing the liquid up using the first stop, and pushed the liquid out using the second stop. A new pipette tip was used for each sample. After they were all filled, we placed them in the OpenPCR for kdfjah;klga. Fluorimeter ProcedureSmart Phone Camera Settings
Data Collection and AnalysisImages of High, Low, and Zero Calf Thymus DNA
Images of Our PCR Negative and Positive Controls
PCR Results: PCR concentrations solved TABLE GOES HERE
PCR Results: Summary
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