BME100 f2014:Group4 L5

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BME 100 Fall 2014 Home
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Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
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OUR TEAM

Name: Anyssa Iwamoto
Name: student
Name: student
Name: student
Name: student
Name: student


LAB 5 WRITE-UP

Procedure

Smart Phone Camera Settings

  • Type of Smartphone: iPhone 5C
    • Flash: Off
    • ISO setting: N/A
    • White Balance: N/A
    • Exposure: N/A
    • Saturation: N/A
    • Contrast: N/A


Calibration

  • Distance between the smart phone cradle and drop = 7.8 cm


Solutions Used for Calibration

row 1 cell 1 row 1 cell 2 row 1 cell 3 row 1 cell 4
row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4
row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4



Placing Samples onto the Fluorimeter

  1. [Instructions: Step one, in your OWN words]
  2. [Instructions: Step two, in your own words]
  3. [Instructions: Step three, in your own words]
  4. [Instructions: Step etc., in your own words]


Data Analysis

Representative Images of Negative and Positive Samples


Image J Values for All Calibrator Samples


TImage J Data

"Tube Label" "Area" "Mean Pixel Value" "RAWINTDEN of Drop" "RAWINTDEN of Background RAWINTEDEN (Drop-Background)
5 - 1 498 238.514 118780 755
5 - 2 498 237.960 118504 1135
5 - 3 498 253.303 126145 1918
2 - 1 row 3 cell 2 row 3 cell 3 row 3 cell 4
2 - 2 row 3 cell 2 row 3 cell 3 row 3 cell 4
2 - 3 row 3 cell 2 row 3 cell 3 row 3 cell 4
row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4
row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4
row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4
row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4
row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4
row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4
row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4
row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4
row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4
row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4
row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4
row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4
row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4


Calibration curve


PCR Results Summary

  • Our positive control PCR result was ____ μg/mL
  • Our negative control PCR result was ____ μg/mL

Observed results

  • Patient _____ :
  • Patient _____ :

Conclusions

  • Patient _____ :
  • Patient _____ :




SNP Information & Primer Design

Background: About the Disease SNP


Primer Design and Testing