BME100 f2014:Group3 L4: Difference between revisions
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| [[Image:SENIORS-11.jpg|100px|thumb|Name: Marissa Seelhammer]] | | [[Image:SENIORS-11.jpg|100px|thumb|Name: Marissa Seelhammer]] | ||
| [[Image: | | [[Image:brianna.jpg|100px|thumb|Name: Brianna Denuit]] | ||
| [[Image: Far.JPG|100px|thumb|Name: Farhad Eghbalian]] | | [[Image: Far.JPG|100px|thumb|Name: Farhad Eghbalian]] | ||
| [[Image:facebook1.jpg|100px|thumb|Name: Shane Mitchell]] | | [[Image:facebook1.jpg|100px|thumb|Name: Shane Mitchell]] |
Latest revision as of 09:15, 29 October 2014
BME 100 Fall 2014 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||
OUR TEAMLAB 4 WRITE-UPProtocolMaterials
DNA Sample Set-up Procedure
Make sure to change the pipette tip after every use to avoid contamination.
Make sure to change the pipette tip after every use to avoid contamination.
Make sure to change the pipette tip after every use to avoid contamination.
OpenPCR program
Bonus
Research and DevelopmentPCR - The Underlying Technology Template DNA is the original strand of DNA used to make copies. Primers are short pieces of DNA that are customized so they can have any sequence of nucleotides. 2 primers are made to match the sequence of DNA that is being copied. Taq Polymerase is an enzyme used in the PCR reaction that acts like a machine and matches the nucleotides with the DNA code making copies. At 95 degrees celsius the DNA double helix is separated into 2 single stranded DNA molecules. It is then cooled down to 50 degrees celsius where the primers work their way in and lock onto their target strands before the strands can reconnect. It is then heated again to 72 degrees celsius where the DNA polymerase are activated and they locate a primer attached to a single DNA stand where it begins to add complementary nucleotides onto the strand. This always occurs when a DNA polymerase molecule hits a primer that is base paired to a longer piece of DNA.
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