BME100 f2014:Group33 L6

BME 100 Fall 2014	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
U-BEAMS Name: Emigdio Ruiz Esquivel Name: John Cunningham Name: Wandasun Sihanath Name: Chris Adams Name: Tim Snelling Name: Breanna Falcon LAB 6 WRITE-UP Bayesian Statistics Overview of the Original Diagnosis System What Bayes Statistics Imply about This Diagnostic Approach Computer-Aided Design TinkerCAD Our Design The design featured incorporates a cradle that is actually attached to the fluorimeter in order to decrease the inconsistency in measurements. This way if the fluroimeter is accidentally nudged than the whole device will move with each other. By attaching the cradle to the fluorimeter, the measurements will be more concise and easy to spot. This gets rid of the annoyance of having to continually measure the distance the light box and cradle were moved, so that the pictures are able to come out more clearly especially since they are needed for processing in ImageJ. The fluorimeter will still be able to be adjusted to ensure that the blue LED light on the fluorimeter illuminates the drop that are placed on the slides. Feature 1: Consumables Kit Weaknesses in the Consumables The consumables were very typical, but how they were packaged created a fussy workplace. The micropipettor tips were nicely placed in an organized tray, but the tray itself had some design issues. Due to an open bottom and loose enclosure of the tips, they were easily knocked out of the holding tray. In a real life experiment, this would be an easy cause for contamination. Feature 2: Hardware - PCR Machine & Fluorimeter Weaknesses of the PCR Machine & Flourimeter Although both the PCR Machine and Flourimeter worked successfully in their own ways, both systems had their own different weaknesses. The PCR Machine was very simple and easy to use and resulted in successful end results. However, the machine could only hold a certain set number of samples because of this the samples took a long time to process in the PCR machine. The Flourimeter itself was very portable and easy to set up anywhere, but because of this, the entire system was very unstable. The camera phone that was placed in the cradle moved at the slightest touch and resulted in inconsistent results and pictures.