Experiment 1:Humans
In our human subjects, when given a 0mg dosage of LPS, the average inflammotin produced in the subjects was 3.834pg/ml with a standard deviation of 1.523 and a standard error of .4816. When given a 5mg dosage of LPS, the average infammotin amount produced was 8.932pg/ml with a standard deviation of 1.5939 and a standard error of .504. When given a 10mg dosage, the average inflammotin amount produced was 61.622pg/ml with a standard deviation of 30.1107 and a standard error of 9.5218. Finally, when given a 15mg dosage, the average protein amount was 657.941pg/ml with a standard deviation of 212.943 and a standard error of 67.338.
For our Bonferonni post hoc test, our p value that we set as our standard was .00083. Our 0 to 5 mg comparison yielded a .000003555 t test value, 0 to 10 yielded .000009944, 0 to 15 yielded .000000014, 5 to 10 yielded .000030186, 5 to 15 yielded .000000016, and 10 to 15 yielded .000000065. All of these values are less than .00083, making our statistics statistically significant.
Experiment 2: Rats
LPS Dose
0
10
Inflammotin (pg/ml)
9.24
22.34
8.76
6.45
8.78
14.23
13.5
3.55
12.3
8.99
Average
10.516
11.112
Standard Deviation
2.2255516
7.4028859
Number Subjects
5
5
Standard Error
0.9952969
3.3106712
The first 5 rats where tested on 0mg of LPS as a control they resulted in an average inflammation of 10.516 with a deviation approximately 2.226 in the
inflammation these resulted in a 0.995296941 standard error in reading. The second set of subjects (5 rats) where tested with a 10 mg dosage of Lipopolysachrid, These test showed an average inflammation of 11.112 which is 0.596 greater than the average of the 0mg subjects, in the 10mg group there was a standard divination of 7.403 between the inflammation level readings, the standard error in readings is 3.311. When the t test was carried on the results the resulting t test value was 0.8674, which points out the probability that the differences will occur if we
ran the experiment again.
The graph bar shows that the effect of LPS dosage on the two subjects does not differ with an amount more than one thus the effect of LPS from 0 to 10mg dosage on rats is not as strong as it is on humans.
Analysis
Experiment 1
Experiment 2
Summary/Discussion
This experiment shows the affect of LPS on level of protein in blood on two different type of subjects. The first subjects were humans which were same or above age of 60 and they were divided in four different groups base on dosage of LPS. By reviewing the results, LPS had a significant affect on protein level in these subjects and also the P-values which are less than 0.00083 shows our statistics are significant.Another type of our subjects were rats which were divided in two different groups base on dosage of LPS. In this experiment, by according to the P-value which was 0.8674, if we ran this experiment again we will get a different results and also by reviewing the results, we can find, the LPS didn't have a strong affect on rats. there for in the second experiment we need to choose more subjects and have more different type of groups of rats.
BME 100 Fall 2014
