BME100 f2014:Group2 L6
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OUR COMPANY
LAB 6 WRITE-UPBayesian StatisticsOverview of the Original Diagnosis System To test the patients for the disease-associated SNP, the labor was divided between thirty-four teams of six students each. These teams worked together to diagnose sixty-eight patients total. Each team diagnosed two patients. There were many precautions taken to prevent error. One precaution was each team tested three replicates per patient. For the PCR controls, SYBR Green I Dye was used for each replication. The same volume of SYBR Green I Dye and DNA in µl was used. For the ImageJ calibration controls, the color channels were separated, and only the green channel was measured. The images were compared to the previously established positive and negative controls. Three images were used for the each unique PCR sample in the ImageJ analysis. After compiling the class data, there were eight inconclusive results. There were six blank tests. There were thirty positive tests and twenty-four negative tests.
What Bayes Statistics Imply about This Diagnostic Approach
The PCR values for calculations one and two are close to 1.00, therefore the PCR replicates are reliable in concluding if a patient has the disease SNP or not. There are many sources of error that could have occurred that could have affected the Bayes values in a negative way. One possible source of human error is the sample preparer contaminating the patient’s PCR sample with their own DNA. Another possible human error is the mislabeling of the positive and negative PCR controls which would have negatively affected all the ImageJ analysis. A possible source of machine error is the PCR machine getting stuck which would prevent the tester from knowing if the PCR reaction was properly completed. The PCR values for calculations three and four are not close to 1.00. Thus, the PCR is not reliable in the predicting of the development of the specific disease SNP, also referred to as diagnosis. Computer-Aided DesignTinkerCAD Tinkercad is a web-based 3D design application. Tinkercad let's you design products with all the work done and saved on the lab. A Tinkercad account is not needed to design these products. This software was used to design a 3D representation of an improved PCR machine. Using Tinkercad, the different pieces of the PCR machine were assembled into a single 3D design.
Feature 1: Consumables KitFeature 2: Hardware - PCR Machine & Fluorimeter |