[Instructions: Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.]
Place the camera in the cradle and set it 4cm away from the fluorimeter. Place plates under the fluorimeter so that when looking through the camera the slide is at eye level and the back of the slide can not be seen.
Solutions Used for Calibration
Initial Concentration of 2X Calf Thymus DNA Solution (micrograms/mL)
Volume of the 2X DNA Solution (μL)
Volume of SYBR GREEN I Dye Solution (μL)
Final DNA Concentration in SYBR Green I Solution (μg/mL)
5
80
80
2.5
2
80
80
1
1
80
80
0.5
0.5
80
80
0.25
0.25
80
80
0.125
0
80
80
0
Placing Samples onto the Fluorimeter
Step one, Turn the fluorimeter light on and pull the slide so that the light shines in the middle of two dots on the slide
Step two, Get a new tip on the micro pipette and place 80mL of the SYBR Green I on the slide in between two dots on the slide. Dispose of this tip in the plastic cup .
Step three, Replace micro pipette with a new tip and place 80mL of the DNA sample onto the SYBR Green I dot
Step four, Set camera timer and close the box so that no light can enter when the timer goes off and the picture is taken. Take three pictures this way.
Step five, Remove the sample from the slide using the micro pipette and dispose of it in a plastic cup along with the tip
Step six, Push slide so that the next row of dots is in the lights pathway and complete steps two through five using your next desired DNA sample
BME 100 Fall 2014
