BME100 f2014:Group28 L5: Difference between revisions
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{| style="wikitable" width="700px" | {| style="wikitable" width="700px" | ||
|- valign="top" | |- valign="top" | ||
| [[Image:BME103student.jpg|100px|thumb|Name: | | [[Image:BME103student.jpg|100px|thumb|Name: student]] | ||
| [[Image:BME103student.jpg|100px|thumb|Name: | | [[Image:BME103student.jpg|100px|thumb|Name: student]] | ||
| [[Image:BME103student.jpg|100px|thumb|Name: | | [[Image:BME103student.jpg|100px|thumb|Name: student]] | ||
| [[Image:BME103student.jpg|100px|thumb|Name: | | [[Image:BME103student.jpg|100px|thumb|Name: student]] | ||
| [[Image:BME103student.jpg|100px|thumb|Name: | | [[Image:BME103student.jpg|100px|thumb|Name: student]] | ||
| [[Image:BME103student.jpg|100px|thumb|Name: | | [[Image:BME103student.jpg|100px|thumb|Name: student]] | ||
|} | |} | ||
<!-- Note: Delete any image placeholders that you do not need. --> | <!-- Note: Delete any image placeholders that you do not need. --> | ||
=LAB 5 WRITE-UP= | =LAB 5 WRITE-UP= | ||
==Procedure== | ==Procedure== | ||
'''Smart Phone Camera Settings'''<br> | '''Smart Phone Camera Settings'''<br> | ||
<!-- The type of smart phone you used and how you adjusted the camera settings, if applicable. If you used more than one phone, make an additional list. --> | |||
<!-- | |||
* Type of Smartphone: | * Type of Smartphone: | ||
** Flash: | ** Flash: | ||
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'''Calibration'''<br> | '''Calibration'''<br> | ||
<!-- | <!-- INSTRUCTIONS: In the space below, briefly describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points. --> | ||
<!-- INSTRUCTIONS: Type the distance between your phone cradle and the drop after the equal sign. --> | |||
* Distance between the smart phone cradle and drop = | * Distance between the smart phone cradle and drop = | ||
'''Solutions Used for Calibration''' | '''Solutions Used for Calibration''' | ||
{| {{table}} width=700 | {| {{table}} width=700 | ||
|- | |- | ||
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| row 3 cell 1 || row 3 cell 2 || row 3 cell 3 || row 3 cell 4 | | row 3 cell 1 || row 3 cell 2 || row 3 cell 3 || row 3 cell 4 | ||
|} | |} | ||
<!-- Add more rows | |||
<!-- Add more rows and cells as needed. --> | |||
'''Placing Samples onto the Fluorimeter''' | '''Placing Samples onto the Fluorimeter''' | ||
# ''[Instructions: Step one, in your OWN words]'' | |||
# Step | # ''[Instructions: Step two, in your own words]'' | ||
# Step | # ''[Instructions: Step three, in your own words]'' | ||
# Step | # ''[Instructions: Step etc., in your own words]'' | ||
<br> | <br> | ||
<!-- Note: Be sure to delete the instruction text in brackets: ''[ ]'' --> | |||
==Data Analysis== | ==Data Analysis== | ||
'''Representative Images of Samples''' | '''Representative Images of Negative and Positive Samples''' | ||
<!-- | <!-- INSTRUCTIONS: (1) Show ONE image where you drew a circle around the droplet with the freehand tool for any sample with *no* DNA. (2) Show ONE image where you drew a circle around the droplet with the freehand tool for a sample *with* DNA (positive signal). -If you include more than two images, you will not receive any additional credit. --> | ||
'''Image J Values for All Samples''' | '''Image J Values for All Calibrator Samples''' | ||
<!-- | <!-- INSTRUCTIONS: Show a table for the ImageJ calf thymus DNA data. '''To save time on typing a new Wiki table from scratch''', use THIS TOOL to auto-generate a Wiki table: http://excel2wiki.net/wikipedia.php. Copy the headers and values from the Excel spreadsheet you made, paste them into the form field, click submit, copy the Wiki code that the tool generated, and replace TABLE GOES HERE (below) with your auto-generated code. --> | ||
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''' | '''Calibration curve'''<br> | ||
<!-- | <!-- INSTRUCTIONS: Place an image of your Excel plot with a line of best fit here. --> | ||
'''PCR Results Summary''' | |||
<!-- INSTRUCTIONS: You completed 8 PCR reactions and used the SYBR Green I staining and imaging technique to measure the amount of amplified DNA in each PCR reaction. You used a standard curve (based on known concentrations of calf thymus DNA) to convert INTDEN values into DNA concentration. Your positive control and negative control samples should be used as '''threshold''' values for determining whether an unknown (patient) sample is truly positive or negative. Replace the underscore with your claculated initial concentration values.--> | |||
* Our positive control PCR result was ____ μg/mL | |||
* Our negative control PCR result was ____ μg/mL | |||
<u>Observed results</u> | |||
<!-- INSTRUCTIONS: Replace the underscore with each patient ID. After the colon, write both a qualitative (what the images looked like) and a quantitative description (μg/mL) of what you observed --> | |||
* Patient _____ : | |||
* Patient _____ : | |||
<u>Conclusions</u> | |||
<!-- INSTRUCTIONS: Compare each patient's results to the positive control value and the negative control value. Draw a final conclusion for each patient (positive or negative) and explain why you made that conclusion. --> | |||
* Patient _____ : | |||
* Patient _____ : | |||
<br><br> | |||
==SNP Information & Primer Design== | |||
'''Background: About the Disease SNP''' | |||
<!-- INSTRUCTIONS: This content is from PCR Lab D. Write a summary, at least five sentences long, about the disease SNP in your own words. --> | |||
'''Primer Design and Testing''' | |||
<!-- INSTRUCTIONS: Write a short summary of the results of your primer test. Underneath your summary, include a screen capture of the results web page. You may crop the image so that it only includes the relevant information. --> | |||
<!-- Do not edit below this line --> | <!-- Do not edit below this line --> | ||
|} | |} |
Revision as of 10:03, 29 October 2014
BME 100 Fall 2014 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||||
OUR TEAM
LAB 5 WRITE-UPProcedureSmart Phone Camera Settings
Data AnalysisRepresentative Images of Negative and Positive Samples
Image J Values for All Calibrator Samples
Observed results
Conclusions
SNP Information & Primer DesignBackground: About the Disease SNP
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