BME100 f2014:Group23 L6: Difference between revisions
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The design of the PCR machine in terms of materials, with the wood exterior being durable, lightweight, and fairly resistant to any heat produced, was kept the same. Likewise, the | The design of the PCR machine in terms of materials, with the wood exterior being durable, lightweight, and fairly resistant to any heat produced, was kept the same. Likewise, the overall structure of the PCR was kept the same. Instead of redesigning the PCR machine, the flourimeter was attached to the side of the PCR machine. The flourimeter consists of a rectangular box with four level. The side of the box can open so that slide may be placed inside. A camera will come in the package, attached by an adjustable camera holder that may slide up and down to take pictures of the droplets. This design is ideal because all of the work can be done in one space. Additionally, a software to analyze the images will be included in the package. This way, by hooking up the camera to the computer and transferring the images to the computer, the software will automatically analyze the images and come up with a positive or negative result, so long as each | ||
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Revision as of 16:30, 25 November 2014
BME 100 Fall 2014 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | ||||||
OUR COMPANY
LAB 6 WRITE-UPBayesian StatisticsOverview of the Original Diagnosis System For testing the 68 patients each of the 34 teams of 6 students tested 2 patients. For each patient 3 DNA samples were taken to avoid any coincidental errors. Each team also had positive and negative controls, as well as different concentrations of the DNA sample in order to be able to analyze whether each patient sample was positive or negative. Three images of each concentration and each patient sample and positive and negative control samples were taken, so as to prevent error. In order to set up the fluorimeter a camera was leveled to each sample drop combined with SYBR green. These images were analyze for numerical values of green INTDEN using ImageJ, since green fluorescence determines the prescence of the disease. These numerical values allowd us to analyze the concentrations of the patient samples and compare them to the controls. If all 3 or 2/3 samples for each patient where determined to be positive (that means within the positive concentration range) then the teams concluded a positive result for that patient, and so with negative results. After analyzing each image, 8 patients received an inconclusive test result, while 30 patients received a positive test result, and 24 patients received a negative test result. Additionally, 3 groups did not complete the test analysis, so 6 patients did not receive any results.
Calculations 1 and 2 show that the system is not perfect, but still reliable for testing this disease. Both specificity and sensitivity calculations were close to 100%, and were at worst 23% from being perfectly accurate. As for human error there could be errors in concentrations in the drops for the fluorimeter. Another possible human error could be cross-contamination by using the same tips while transferring different samples. Another possible error, both human and machine, could be faulty INTDEN values from the ImageJ software. This could be due to wrong placement of ovals for the measurements.
Computer-Aided DesignName of device: PCRF- Polymerase Chain Reaction Fluorimeter
TinkerCAD Our Design Device Description
Feature 1: Consumables KitThe SYBR GREEN 1 Solution, buffer, Calf Thymus DNA, flourimeter slides, positive and negative samples, pipette tips, and plastic test tubes will be placed in a dark cold box. All components will be wrapped in bubble wrap as a precaution. Feature 2: Hardware - PCR Machine & FluorimeterThe design of the PCR machine in terms of materials, with the wood exterior being durable, lightweight, and fairly resistant to any heat produced, was kept the same. Likewise, the overall structure of the PCR was kept the same. Instead of redesigning the PCR machine, the flourimeter was attached to the side of the PCR machine. The flourimeter consists of a rectangular box with four level. The side of the box can open so that slide may be placed inside. A camera will come in the package, attached by an adjustable camera holder that may slide up and down to take pictures of the droplets. This design is ideal because all of the work can be done in one space. Additionally, a software to analyze the images will be included in the package. This way, by hooking up the camera to the computer and transferring the images to the computer, the software will automatically analyze the images and come up with a positive or negative result, so long as each |