BME100 f2014:Group21 L2

BME 100 Fall 2014	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Dustin D. Burley Name: Mackenzie Konves Name: Scott Boege Name: Nicholas Goettl Name: Travis Tibbs Name: Lionel Davis LAB 2 WRITE-UP Descriptive Statistics Experiment 1 Will be the effect of LPS on rats? MEAN, STANDARD DEVIATION, STANDARD ERROR OF THE EXPERIMENT ON RATS There was almost no change in the levels of Inflammotin in the rats. The difference between the mean of the 0mg group and the 10mg group is 0.6. This could possibly be because rats require a dosage of LPS higher than 10mg, or that the test conducted was not done properly. Experiment 2 Will be the effect of LPS on humans? MEAN, STANDARD DEVIATION, STANDARD ERROR OF THE EXPERIMENT ON HUMANS There was a strong positive correlation between the different dosages of LPS and the production of Inflammotin. The relationship is nearly exponential. Results Experiment 1 The results showed that the the dosage of LPS on rats didn't do much on their levels of inflammotin, or that the tests done on the rats were not done properly. ‎Experiment 2 Analysis Experiment 1 A one-way t-test was chosen for this experiment since only two groups are present. Since the p-value(0.867403) of the t-test is greater than 0.05, we do not have statistical evidence that there is a difference in inflammotin production between the group that received a 0mg dose and the group that received a 10mg dose. Experiment 2 ANOVA test for human inflammotin A one-way ANOVA test was chosen for this experiment in order to investigate the differences among more than two groups. Since the p-value(1E-16) of the ANOVA test is less than 0.05, we can determine that there is a statistical difference in the levels of inflammotin found in humans among those who did not take LPS, those who took 5mg of LPS, those who took 10mg of LPS, and those who took 15mg of LPS. Summary/Discussion An experiment was performed on a group of rats and subsequently, a group of elderly people living in a retirement home. From the experiment, it was initially concluded that the dosage of Inflammotin did not affect the protein levels of the rats; the variation between the 0 and 10mg doses was 0.6 pg/ml. A one-way t-test was performed to quantify the differences between the two groups of rats and validate the results. This test proved the study to be inconclusive because the small amount of data showed no quantifiable differences. Unlike the rat experiment, an effect was observed in human subjects; the average production of Inflammotin between the 0, 5, 10, and 15mg dosages of LPS increased exponentially. Furthermore, an ANOVA test was performed to evaluate differences between the four human groups. This test validated the results proving that higher dosages of LPS caused increased production of Inflammotin in humans, as the p-value was less than 0.05. In conclusion, this experiment proved the dosages of LPS affected the production of Inflammotin in humans but proved inconclusive in rats.