BME100 f2014:Group1 L5

BME 100 Fall 2014	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Hawley Helmbrecht Name: Timothy Black Name: Sarah Fakhoury Role(s) Name: Prerna Gupta Role(s) Name: Jonathan Riecker Name: Michael Pineda Role(s) LAB 5 WRITE-UP Procedure Smart Phone Camera Settings Type of Smartphone: Samsung S3 Flash: No Flash ISO setting: 800 White Balance: Auto Exposure: +2.0 Saturation: No setting to affect Saturation Contrast: Auto Contrast Off Calibration Place the phone in the cradle with the camera lens lined up so that it shows a view of the slide from the side (edge-on). Once the camera is set up in the cradle and lined up with the side of the slide, adjust the slide so that the light illuminates the center of the drop and the drop focuses light on the other side of it. After adjusting the slide itself, change the distance of the smartphone from the fluorimeter in order to keep the drop in focus but keep the view close. Distance between the smart phone cradle and drop = 5 cm Solutions Used for Calibration PCR Product Label Tube Volume of the DILUTED PCR Product Solution (μL) Volume of the SYBR GREEN I Dye Solution (μL) Dilution 1 Dilution 2 Total Dilution (Simplified Fraction) Positive 80 80 1/6 1/2 1/12 Negative 80 80 1/6 1/2 1/12 G1 1-1 80 80 1/6 1/2 1/12 G1 1-2 80 80 1/6 1/2 1/12 G1 1-3 80 80 1/6 1/2 1/12 G1 2-1 80 80 1/6 1/2 1/12 G1 2-2 80 80 1/6 1/2 1/12 G1 2-3 80 80 1/6 1/2 1/12 Placing Samples onto the Fluorimeter Find the rough side of the slide (making sure to only touch the slide while wearing gloves) Place the slide, with the rough side up, in the fluorimeter Locate the two clear circles in the middle of the slide and add 80 μL of SYBR Green I solution On the same circle with the SYBR Green I solution, place 80 μL of Sample/Calibration drops Adjust the slide so that the middle of the drop is illuminated by the light and the drop becomes focused on the side Data Analysis Representative Images of Negative and Positive Samples Image J Values for All Calibrator Samples Concentration of 2X Calf Thymus DNA solution(micrograms/mL) Volume of the 2X DNA solution (μL) Volume of the SYBR GREEN I Dye Solution (μL) Final DNA concentration in SYBR Green I solution (μg/mL) 5 80 80 2.5 2 80 80 1 1 80 80 0.5 0.5 80 80 0.25 0.25 80 80 0.125 0 80 80 0 Solutions Used for Calibration Calibration curve PCR Results Summary Our positive control PCR result was ____ μg/mL Our negative control PCR result was ____ μg/mL Observed results Patient _____ : Patient _____ : Conclusions Patient _____ : Patient _____ : SNP Information & Primer Design Background: About the Disease SNP Primer Design and Testing include a screen capture of the results web page