BME100 f2014:Group1 L5: Difference between revisions

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| [[Image:Michael's_Photo.jpg|100px|thumb|Name: Michael Pineda<br>Role(s)]]
| [[Image:Michael's_Photo.jpg|100px|thumb|Name: Michael Pineda<br>Role(s)]]
|}
|}
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=LAB 5 WRITE-UP=
=LAB 5 WRITE-UP=
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'''Smart Phone Camera Settings'''<br>
'''Smart Phone Camera Settings'''<br>
<!-- The type of smart phone you used and how you adjusted the camera settings, if applicable. If you used more than one phone, make an additional list. -->
<!-- The type of smart phone you used and how you adjusted the camera settings, if applicable. If you used more than one phone, make an additional list. -->
* Type of Smartphone:Samsung Galaxy S3
* Type of Smartphone:
** Flash: No Flash
** Flash:
** ISO setting:800
** ISO setting:
** White Balance: Auto
** White Balance:  
** Exposure: +2.0
** Exposure:
** Saturation: Not an option on this phone camera
** Saturation:
** Contrast: Auto Contrast off
** Contrast:




'''Calibration'''<br>
'''Calibration'''<br>
<!-- INSTRUCTIONS: In the space below, riefly describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points. -->


''[Instructions: Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.]''
<!-- INSTRUCTIONS: Type the distance between your phone cradle and the drop after the equal sign. -->
 
* Distance between the smart phone cradle and drop =
* Distance between the smart phone cradle and drop =
''[Instructions: See worksheet page 6.]''




'''Solutions Used for Calibration''' ''[Instructions: See worksheet page 6.]''
'''Solutions Used for Calibration'''
{| {{table}} width=700
{| {{table}} width=700
|-
|-
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| row 3 cell 1 || row 3 cell 2 || row 3 cell 3 || row 3 cell 4
| row 3 cell 1 || row 3 cell 2 || row 3 cell 3 || row 3 cell 4
|}
|}
''[Add more rows as needed]''
 
<!-- Add more rows and cells as needed. -->
 




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* Patient _____ :
* Patient _____ :
* Patient _____ :
* Patient _____ :
<br><br>
==SNP Information & Primer Design==
'''Background: About the Disease SNP'''
<!-- INSTRUCTIONS: This content is from PCR Lab D. Write a summary, at least five sentences long, about the disease SNP in your own words. -->
'''Primer Design and Testing'''
<!-- INSTRUCTIONS: Write a short summary of the results of your primer test. Underneath your summary, include a screen capture of the results web page. You may crop the image so that it only includes the relevant information. -->





Revision as of 09:28, 29 October 2014

BME 100 Fall 2014 Home
People
Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
Course Logistics For Instructors
Photos
Wiki Editing Help


OUR TEAM

Name: Hawley Helmbrecht
Name: Timothy Black
Name: Sarah Fakhoury
Role(s)
Name: Prerna Gupta
Role(s)
Name: Jonathan Riecker
Name: Michael Pineda
Role(s)


LAB 5 WRITE-UP

Procedure

Smart Phone Camera Settings

  • Type of Smartphone:
    • Flash:
    • ISO setting:
    • White Balance:
    • Exposure:
    • Saturation:
    • Contrast:


Calibration

  • Distance between the smart phone cradle and drop =


Solutions Used for Calibration

row 1 cell 1 row 1 cell 2 row 1 cell 3 row 1 cell 4
row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4
row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4



Placing Samples onto the Fluorimeter

  1. [Instructions: Step one, in your OWN words]
  2. [Instructions: Step two, in your own words]
  3. [Instructions: Step three, in your own words]
  4. [Instructions: Step etc., in your own words]


Data Analysis

Representative Images of Negative and Positive Samples


Image J Values for All Calibrator Samples


TABLE GOES HERE


Calibration curve


PCR Results Summary

  • Our positive control PCR result was ____ μg/mL
  • Our negative control PCR result was ____ μg/mL

Observed results

  • Patient _____ :
  • Patient _____ :

Conclusions

  • Patient _____ :
  • Patient _____ :




SNP Information & Primer Design

Background: About the Disease SNP


Primer Design and Testing