BME100 f2014:Group18 L6

BME 100 Fall 2014	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR COMPANY Name: Norah Alkhamis Name: Jesus Calderon Name: Kevin Couch Name: Jordan Kariniemi Name: Scott Slade Name: Rachel Tomlinson LAB 6 WRITE-UP Bayesian Statistics Overview of the Original Diagnosis System To begin, each group in the BME 100 class was given two patients, and instructed to test these patients for the disease-associated SNP. The total laboratory consisted of 34 teams (consisting of 6 students each team) with two patients assigned to each team. All together, there were 68 patients that were being tested for the disease. Originally, while testing the droplets of DNA with the fluorimeter, each testing sample had three pictures taken of it under the light of the fluorimeter. These images were then grouped, analyzed, and then the data was averaged in hopes of finding the most accurate data for each specific sample. Each patient had three replicants of their DNA tested for the disease. The mean of this data was then taken to continuously potentially limit any error. The BME 100 class data was comprised into a master spreadsheet that included all of the teams' work/data. The data across the groups varied. Some groups' data had successfully concluded their sample data, while others received inconclusive results, or left their information blank. Possible sources of error may be due to the multitude of teams in the whole BME 100 class. Because there were 34 teams, this suggested that there were 34 different ways to perform the lab, meaning the data could have been received in a different manner each time. What Bayes Statistics Imply about This Diagnostic Approach Computer-Aided Design TinkerCAD Using the TinkerCAD program, our group was able to refer to and analyze the standard open PCR machine in 3-D to develop an idea for what ways it could be improved. With each idea of improvement, the implications of the change were discussed. For example, we considered if the PCR machine was designed to be able to hold more samples, the process would take longer, and most likely need another heating/cooling element, thus causing a need for a larger powered circuit board. In the end, we decided as a group that we should add another cooling element to the existing PCR design. This would not significantly change the design of the PCR machine but it would allow for the final PCR reaction to be reached quicker and more efficiently. Our Design We added a cooling element to the Open PCR machine. The process for the PCR reaction requires a cooling stage, and this added element allows for the test tube samples to reach the cool temperature in shorter time and more efficiently. Ultimately, it is reducing the total time required for the PCR reaction. Feature 2: Consumables Kit We will hold the SYBR Green 1 in light blocking packaging which would replace the original tin foil used in the lab. The packaging would also be water proof to protect from cross contamination. The buffers needed to be sterile to ensure the cleanliness and accuracy of the lab. The packaging for the micropippeor tips could be cut in half that way they could all be used in one sitting for the lab and that would allow for the most sterile process. Feature 3: Hardware - PCR Machine & Fluorimeter For the fluorimeter we would include an adjustable camera holder that will allow for varying heights to have the camera flush with the side of the machine. We would also change the folding lid of the fluorimeter that way it allow for complete darkness, but still allow for a clear image. The lack of these two features in the existing fluorimeter design that we used caused some problems. Without the adjustable camera holder, each change in camera while testing to see who's worked better, the entire fluorimeter set up had to be redone. Also, lowering the flap with the camera on timer, caused glare and problems due to the camera adjusting to the light. In order to get a clear picture, the lab had to be slightly compromised and allowed to the flap to be open partially allowing some light to shine into the fluorimeter.