Data Analysis
Representative Images of Negative and Positive Samples
POSITIVE CONTROL IMAGE
NEGATIVE CONTROL IMAGE
Image J Values for All Calibrator Samples
EXCEL Table1 RAW DATA / Drop-Background
DNA Solution
|
Area
|
Pixel Density
|
RAWINTDEN DROP
|
RAWINTDEN BACK
|
RAWINTDEN DROP-BACK
|
0 |
58604 |
61.036 |
3576976 |
175112 |
3401864
|
0.25 |
62324 |
88.828 |
5536096 |
175120 |
5360976
|
0.5 |
57291 |
108.156 |
6196361 |
165186 |
6031175
|
1 |
63052 |
152.1 |
9590219 |
189151 |
9401068
|
2 |
54628 |
195.326 |
10670272 |
140838 |
10529434
|
5 |
53200 |
222.41 |
11832237 |
144816 |
11687421
|
Positive-1 |
56188 |
199.791 |
11225837 |
138134 |
11087703
|
Positive-2 |
57469 |
62.39 |
11228718 |
140106 |
11088612
|
Positive-3 |
59569 |
59.235 |
11231472 |
142363 |
11089109
|
Negative-1 |
57504 |
76.644 |
4407348 |
132161 |
4275187
|
Negative-2 |
59222 |
61.73 |
4409466 |
134890 |
4274576
|
Negative-3 |
62109 |
59.235 |
4410288 |
137715 |
4272573
|
Patient 1.Trial 1-1 |
65920 |
86.368 |
5693383 |
169276 |
5524107
|
Patient 1.Trial 1-2 |
66209 |
60.88 |
5693737 |
169817 |
5523920
|
Patient 1.Trial 1-3 |
69148 |
60.235 |
5694930 |
170726 |
5524204
|
Patient 1.Trial 2-1 |
56864 |
73.951 |
4205157 |
142168 |
4062989
|
Patient 1.Trial 2-2 |
59667 |
62.3 |
4205869 |
143593 |
4062276
|
Patient 1.Trial 2-3 |
62540 |
61.235 |
4206231 |
145447 |
4060784
|
Patient 1.Trial 3-1 |
62180 |
69.03 |
4292263 |
149505 |
4142758
|
Patient 1.Trial 3-2 |
63625 |
63.36 |
4292430 |
151453 |
4140977
|
Patient 1.Trial 3-3 |
64252 |
62.235 |
4292505 |
152708 |
4139797
|
Patient 2.Trial 1-1 |
43144 |
230.772 |
9956425 |
177344 |
9779081
|
Patient 2.Trial 1-2 |
43675 |
65.03 |
9958551 |
179669 |
9778882
|
Patient 2.Trial 1-3 |
45197 |
63.235 |
9960640 |
180648 |
9779992
|
Patient 2.Trial 2-1 |
48880 |
212.619 |
10392841 |
146922 |
10245919
|
Patient 2.Trial 2-2 |
49197 |
64.32 |
10395760 |
148476 |
10247284
|
Patient 2.Trial 2-3 |
50275 |
64.235 |
10395955 |
150674 |
10245281
|
Patient 2.Trial 3-1 |
48880 |
212.619 |
10392841 |
121932 |
10270909
|
Patient 2.Trial 3-2 |
49023 |
62.34 |
10392996 |
121968 |
10271028
|
Patient 2.Trial 3-3 |
50014 |
65.235 |
10395265 |
122717 |
10272548
|
EXCEL Table 2 Edited to included background-subtractions
Final DNA Concentraion in SYBR Green 1 Solution (microgram/mL)
|
RAWINTDEN DROP-BACK
|
|
|
|
Standard Deviation
|
|
1 |
2 |
3 |
MEAN |
|
0 |
3401864 |
3401646 |
3401953 |
3401821 |
158
|
0.25 |
5360976 |
5360273 |
5358990 |
5360080 |
1007
|
0.5 |
6031175 |
6030785 |
6030810 |
6030923 |
218
|
1 |
9401068 |
9403417 |
9403330 |
9402605 |
1332
|
2 |
10529434 |
10530484 |
10531046 |
10530321 |
818
|
5 |
11687421 |
11687594 |
11687335 |
11687450 |
132
|
Positive |
11087703 |
11088612 |
11089109 |
11088475 |
713
|
Negative |
4275187 |
4274576 |
4272573 |
4274112 |
1367
|
Patient 1.Trial 1 |
5524107 |
5523920 |
5524204 |
5524077 |
144
|
Patient 1.Trial 2 |
4062989 |
4062276 |
4060784 |
4062016 |
1125
|
Patient 1.Trial 3 |
4142758 |
4140977 |
4139797 |
4141177 |
1491
|
Patient 2.Trial 1 |
9779081 |
9778882 |
9779992 |
9779318 |
592
|
Patient 2.Trial 2 |
10245919 |
10247284 |
10245281 |
10246161 |
1023
|
Patient 2.Trial 3 |
10270909 |
10271028 |
10272548 |
10271495 |
914
|
PCR Prod Label
|
Mean RAWINTDEN Drop-Background
|
PCR Product Concentration (ug/mL)
|
Intial PCR Product Concentration (ug/mL)
|
cell |
cell |
cell |
cell
|
cell |
cell |
cell |
cell
|
cell |
cell |
cell |
cell
|
cell |
cell |
cell |
cell
|
cell |
cell |
cell |
cell
|
cell |
cell |
cell |
cell
|
Calibration curve
PCR Results Summary
- Our positive control PCR result was ____ μg/mL
- Our negative control PCR result was ____ μg/mL
Observed results
- Patient _____ :
- Patient _____ :
Conclusions
- Patient _____ :
- Patient _____ :
SNP Information & Primer Design
Background: About the Disease SNP
Primer Design and Testing