BME100 f2014:Group16 L5

BME 100 Fall 2014	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Christopher Saar Protocol editer image Source:smashbroslawlorigins.wikia.com Name: Gurpaul Sidhu Role(s): Wiki editor, researcher Picture Source:www. shadyrecords.com/wp-content/uploads/2013/01/royce.jpg Name: Emily Angeles Mancinas Role: assistant Picture Source:http://wallpaperswa.com Name: student Name: student Name: Romann Arizmendi Assistant LAB 5 WRITE-UP Procedure Smart Phone Camera Settings Type of Smartphone: Flash: NO Flash ISO setting:800 White Balance: Auto Exposure: Zero Saturation:Standard Contrast:Auto Calibration Distance between the smart phone cradle and drop = 6.5 cm Solutions Used for Calibration Init. Conc. 2X Calf Thymus DNA (micrograms/mL) Vol. 2X DNA solution (micro-mL) Vol. SYBR GREEN 1 DYE (micro-mL) Final DNA Concentration STBR GREEN 1 Solution (Micrograms/mL) 5 80 80 row 2 cell 4 2 80 80 row 3 cell 4 1 80 80 row 1 cell 4 0.5 80 80 row 2 cell 4 0.25 80 80 row 3 cell 4 0 80 80 row 3 cell 4 Placing Samples onto the Fluorimeter Place fluorimeter on the table Place slide in the fluorimeter with the smooth side down hydrophobic side up Adjust slide to the light shines between the dots on the slide Place 80 micro-mL of SYBR green 1 on slide between the middle dots Place 80 micro-mL of calibration on the sphere you already placed to a 160 micro-mL shpere Adjust the shpere placement for the light and phone distance in the holder Record Iphone distance Cover the assembly and make small adjustments Take a photo on delay and cover Send photo to be recorded for ImageJ analysis Remove the 160 micro-mL Discard the slide Repeat steps 1-12 Data Analysis Representative Images of Negative and Positive Samples Image J Values for All Calibrator Samples EXCEL Table1 RAW DATA Final DNA Concentration in SYBR Green 1 solution (microgram/mL) Area Mean Pixel Value RAWINTDEN OF THE DROP RAWINTDEN OF THE BACKGROUND 0 Cell Cell Cell Cell 0.25 Cell Cell Cell Cell 0.5 Cell Cell Cell Cell 1 Cell Cell Cell Cell 2 Cell Cell Cell Cell N Cell Cell Cell Cell P Cell Cell Cell Cell 1.1 Cell Cell Cell Cell 1.2 Cell Cell Cell Cell 1.3 Cell Cell Cell Cell 2.1 Cell Cell Cell Cell 2.2 Cell Cell Cell Cell 2.3 Cell Cell Cell Cell EXCEL Table 2 Edited to included background-subtractions Final DNA Concentration in SYBR Green 1 solution (microgram/mL) Area Mean Pixel Value RAWINTDEN OF THE DROP RAWINTDEN OF THE BACKGROUND RAWINTDEN-DROP Background Cell Cell Cell Cell Cell Cell Cell Cell Cell Cell Cell Cell Cell Cell Cell Cell Cell Cell Cell Cell Cell Cell Cell Cell Cell Cell Cell Cell Cell Cell Calibration curve PCR Results Summary Our positive control PCR result was ____ μg/mL Our negative control PCR result was ____ μg/mL Observed results Patient _____ : Patient _____ : Conclusions Patient _____ : Patient _____ : SNP Information & Primer Design Background: About the Disease SNP Primer Design and Testing