BME100 f2014:Group16 L5

BME 100 Fall 2014	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Christopher Saar Protocol editer image Source:smashbroslawlorigins.wikia.com Name: Gurpaul Sidhu Role(s): Wiki editor, researcher Picture Source:www. shadyrecords.com/wp-content/uploads/2013/01/royce.jpg Name: Emily Angeles Mancinas Role: assistant Picture Source:http://wallpaperswa.com Name: Sheania Morgan Roles: Conductor of experiment image source: http://www.iemoji.com/view/emoji/814/places/moyai Name: student Name: Romann Arizmendi Assistant LAB 5 WRITE-UP Procedure Smart Phone Camera Settings Type of Smartphone: Flash: NO Flash ISO setting:800 White Balance: Auto Exposure: Zero Saturation:Standard Contrast:Auto Calibration Distance between the smart phone cradle and drop = 6.5 cm Solutions Used for Calibration Init. Conc. 2X Calf Thymus DNA (micrograms/mL) Vol. 2X DNA solution (micro-mL) Vol. SYBR GREEN 1 DYE (micro-mL) Final DNA Concentration STBR GREEN 1 Solution (Micrograms/mL) 5 80 80 row 2 cell 4 2 80 80 row 3 cell 4 1 80 80 row 1 cell 4 0.5 80 80 row 2 cell 4 0.25 80 80 row 3 cell 4 0 80 80 row 3 cell 4 Placing Samples onto the Fluorimeter Place fluorimeter on the table Place slide in the fluorimeter with the smooth side down hydrophobic side up Adjust slide to the light shines between the dots on the slide Place 80 micro-mL of SYBR green 1 on slide between the middle dots Place 80 micro-mL of calibration on the sphere you already placed to a 160 micro-mL shpere Adjust the shpere placement for the light and phone distance in the holder Record Iphone distance Cover the assembly and make small adjustments Take a photo on delay and cover Send photo to be recorded for ImageJ analysis Remove the 160 micro-mL Discard the slide Repeat steps 1-12 Data Analysis Representative Images of Negative and Positive Samples Positive Sample Negative Sample Image J Values for All Calibrator Samples EXCEL Table1 RAW DATA / Drop-Background DNA Solution Area Pixel Density RAWINTDEN DROP RAWINTDEN BACK RAWINTDEN DROP-BACK 0 58604 61.036 3576976 175112 3401864 0.25 62324 88.828 5536096 175120 5360976 0.5 57291 108.156 6196361 165186 6031175 1 63052 152.1 9590219 189151 9401068 2 54628 195.326 10670272 140838 10529434 5 53200 222.41 11832237 144816 11687421 Positive-1 56188 199.791 11225837 138134 11087703 Positive-2 57469 62.39 11228718 140106 11088612 Positive-3 59569 59.235 11231472 142363 11089109 Negative-1 57504 76.644 4407348 132161 4275187 Negative-2 59222 61.73 4409466 134890 4274576 Negative-3 62109 59.235 4410288 137715 4272573 Patient 1.Trial 1-1 65920 86.368 5693383 169276 5524107 Patient 1.Trial 1-2 66209 60.88 5693737 169817 5523920 Patient 1.Trial 1-3 69148 60.235 5694930 170726 5524204 Patient 1.Trial 2-1 56864 73.951 4205157 142168 4062989 Patient 1.Trial 2-2 59667 62.3 4205869 143593 4062276 Patient 1.Trial 2-3 62540 61.235 4206231 145447 4060784 Patient 1.Trial 3-1 62180 69.03 4292263 149505 4142758 Patient 1.Trial 3-2 63625 63.36 4292430 151453 4140977 Patient 1.Trial 3-3 64252 62.235 4292505 152708 4139797 Patient 2.Trial 1-1 43144 230.772 9956425 177344 9779081 Patient 2.Trial 1-2 43675 65.03 9958551 179669 9778882 Patient 2.Trial 1-3 45197 63.235 9960640 180648 9779992 Patient 2.Trial 2-1 48880 212.619 10392841 146922 10245919 Patient 2.Trial 2-2 49197 64.32 10395760 148476 10247284 Patient 2.Trial 2-3 50275 64.235 10395955 150674 10245281 Patient 2.Trial 3-1 48880 212.619 10392841 121932 10270909 Patient 2.Trial 3-2 49023 62.34 10392996 121968 10271028 Patient 2.Trial 3-3 50014 65.235 10395265 122717 10272548 EXCEL Table 2 Edited to included background-subtractions Final DNA Concentraion in SYBR Green 1 Solution (microgram/mL) RAWINTDEN DROP-BACK Standard Deviation 1 2 3 MEAN 0 3401864 3401646 3401953 3401821 158 0.25 5360976 5360273 5358990 5360080 1007 0.5 6031175 6030785 6030810 6030923 218 1 9401068 9403417 9403330 9402605 1332 2 10529434 10530484 10531046 10530321 818 5 11687421 11687594 11687335 11687450 132 Positive 11087703 11088612 11089109 11088475 713 Negative 4275187 4274576 4272573 4274112 1367 Patient 1.Trial 1 5524107 5523920 5524204 5524077 144 Patient 1.Trial 2 4062989 4062276 4060784 4062016 1125 Patient 1.Trial 3 4142758 4140977 4139797 4141177 1491 Patient 2.Trial 1 9779081 9778882 9779992 9779318 592 Patient 2.Trial 2 10245919 10247284 10245281 10246161 1023 Patient 2.Trial 3 10270909 10271028 10272548 10271495 914 Calibration curve PCR Results Summary Our positive control PCR result was ____ μg/mL Our negative control PCR result was ____ μg/mL Observed results Patient _____ : Patient _____ : Conclusions Patient _____ : Patient _____ : SNP Information & Primer Design Background: About the Disease SNP Primer Design and Testing