BME100 f2014:Group16 L5: Difference between revisions

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<!-- The type of smart phone you used and how you adjusted the camera settings, if applicable. If you used more than one phone, make an additional list. -->
<!-- The type of smart phone you used and how you adjusted the camera settings, if applicable. If you used more than one phone, make an additional list. -->
* Type of Smartphone:
* Type of Smartphone:
** Flash:
** Flash: '''NO Flash'''
** ISO setting:
** ISO setting:'''Standard Iphone settings'''
** White Balance:  
** White Balance: '''Standard Iphone settings'''
** Exposure:
** Exposure: '''Standard Iphone settings'''
** Saturation:
** Saturation:'''Standard Iphone settings'''
** Contrast:
** Contrast:'''Standard Iphone settings'''




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<!-- INSTRUCTIONS: Briefly describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points. -->
<!-- INSTRUCTIONS: Briefly describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points. -->


* Distance between the smart phone cradle and drop =
* Distance between the smart phone cradle and drop = 6.5 cm




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{| {{table}} width=700
{| {{table}} width=700
|-
|-
| row 1 cell 1 || row 1 cell 2 || row 1 cell 3 || row 1 cell 4
| Init. Conc. 2X Calf Thymus DNA (micrograms/mL) ||Vol. 2X DNA solution (micro-mL)|| Vol. SYBR GREEN 1 DYE (micro-mL) || Final DNA Concentration STBR GREEN 1 Solution (Micrograms/mL)
|-
|-
| row 2 cell 1 || row 2 cell 2 || row 2 cell 3 || row 2 cell 4
| 5 || 80|| 80 || row 2 cell 4
|-
|-
| row 3 cell 1 || row 3 cell 2 || row 3 cell 3 || row 3 cell 4
| 2 || 80 || 80 || row 3 cell 4
|-
| 1 || 80 || 80 || row 1 cell 4
|-
| 0.5 || 80 || 80 || row 2 cell 4
|-
| 0.25 || 80 || 80 || row 3 cell 4
|-
| 0 || 80 || 80 || row 3 cell 4
|}
|}
<!-- Add more rows and cells as needed. -->
<!-- Add more rows and cells as needed. -->



Revision as of 10:23, 29 October 2014

BME 100 Fall 2014 Home
People
Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
Course Logistics For Instructors
Photos
Wiki Editing Help


OUR TEAM

Name: Christopher Saar
Protocol editer
image Source:smashbroslawlorigins.wikia.com
Name: Gurpaul Sidhu
Role(s): Wiki editor, researcher
Picture Source:www. shadyrecords.com/wp-content/uploads/2013/01/royce.jpg
Name: Emily Angeles Mancinas
Name: student
Name: student
Name: student


LAB 5 WRITE-UP

Procedure

Smart Phone Camera Settings

  • Type of Smartphone:
    • Flash: NO Flash
    • ISO setting:Standard Iphone settings
    • White Balance: Standard Iphone settings
    • Exposure: Standard Iphone settings
    • Saturation:Standard Iphone settings
    • Contrast:Standard Iphone settings


Calibration

  • Distance between the smart phone cradle and drop = 6.5 cm


Solutions Used for Calibration

Init. Conc. 2X Calf Thymus DNA (micrograms/mL) Vol. 2X DNA solution (micro-mL) Vol. SYBR GREEN 1 DYE (micro-mL) Final DNA Concentration STBR GREEN 1 Solution (Micrograms/mL)
5 80 80 row 2 cell 4
2 80 80 row 3 cell 4
1 80 80 row 1 cell 4
0.5 80 80 row 2 cell 4
0.25 80 80 row 3 cell 4
0 80 80 row 3 cell 4


Placing Samples onto the Fluorimeter

  1. [Instructions: Step one, in your OWN words]
  2. [Instructions: Step two, in your own words]
  3. [Instructions: Step three, in your own words]
  4. [Instructions: Step etc., in your own words]


Data Analysis

Representative Images of Negative and Positive Samples


Image J Values for All Calibrator Samples


TABLE GOES HERE


Calibration curve


PCR Results Summary

  • Our positive control PCR result was ____ μg/mL
  • Our negative control PCR result was ____ μg/mL

Observed results

  • Patient _____ :
  • Patient _____ :

Conclusions

  • Patient _____ :
  • Patient _____ :




SNP Information & Primer Design

Background: About the Disease SNP


Primer Design and Testing