BME100 f2014:Group14 L6: Difference between revisions
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<!-- Instruction 2: IF your group has decided to redesign the PCR machine and/or Fluorimeter to address any major weakness(es), explain how in an additional paragraph. --> | <!-- Instruction 2: IF your group has decided to redesign the PCR machine and/or Fluorimeter to address any major weakness(es), explain how in an additional paragraph. --> | ||
'''Hardware Includes:''' | |||
# PCR Machine | |||
#Lightbox with Built-in Camera | |||
# Fluorimeter | |||
# USB Cord | |||
# Downloadable Image Processing Program (auto-generates the ImageJ results) | |||
<br> The consumables will still be processed through a PCR machine to denature the DNA. The fluorimeter will still be used to process the image of the DNA to look for any tell-tale signs of disease. Some adjustments made to the PCR machine include a quicker heating process for the PCR machine. The denaturing process is quite lengthy and the machine takes a while to go from the differing temperatures and on numerous cycles. | <br> The consumables will still be processed through a PCR machine to denature the DNA. The fluorimeter will still be used to process the image of the DNA to look for any tell-tale signs of disease. Some adjustments made to the PCR machine include a quicker heating process for the PCR machine. The denaturing process is quite lengthy and the machine takes a while to go from the differing temperatures and on numerous cycles. <br> | ||
The fluorimeter, will come equipped with a built- in camera as well as the stand for the slide that is prepositioned to the correct distance from the camera. This will ensure that the image is taken form the correct location as well as remove potential error from the smartphone camera. Once the images are taken, a USB cord will connect the the fluorimeter to a computer and enter images. A downloadable program will then auto-generate the ImageJ processing, making the process more convenient and time efficient. | The fluorimeter, will come equipped with a built- in camera as well as the stand for the slide that is prepositioned to the correct distance from the camera. This will ensure that the image is taken form the correct location as well as remove potential error from the smartphone camera. Once the images are taken, a USB cord will connect the the fluorimeter to a computer and enter images. A downloadable program will then auto-generate the ImageJ processing, making the process more convenient and time efficient. | ||
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Revision as of 23:42, 25 November 2014
BME 100 Fall 2014 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | ||||||
OUR COMPANY
LAB 6 WRITE-UPBayesian StatisticsOverview of the Original Diagnosis System
Since the results for calculations 1 and 2 were close to being 100%, it implies that the reliability of the device is high. A person is more likely to determine whether they are positive or negative for obtaining the disease. Some sources of error may be that there was a miscalculation when analyzing the fluorescence in an image. In addition, the class results may have been miscalculated as well that led to a miscalculation in our Bayes' formula. Another source of error may have come from the machine itself.
The results for calculations 3 and 4 were very small which infers that the PCR is not very reliable for predicting the development of the disease. This implies that the patient will have a very low chance of diagnosis before the onset of the disease. The same errors as calculations 1 and 2 are the same for calculations 3 and 4. Computer-Aided DesignTinkerCAD TinkerCAD is an online product design service that allowed us to replicate the structure of the OpenPCR machine. Using the building blocks given to us through the website thingiverse.com, we re-created a PCR machine virtually. We then modified this basic structure to incorporate some of the changes we felt would better the device. Our Design Our OpenPCR design made through TinkerCAD
Feature 1: Consumables KitConsumables Kit Includes:
Above is an example of the test tubes with the premeasured 50 μL of the primer. The test tubes will have corresponding labels to prevent any confusion. The example depicts the consumables for the positive sample. Feature 2: Hardware - PCR Machine & FluorimeterHardware Includes:
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