BME100 f2014:Group14 L6: Difference between revisions
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==Feature 1: Consumables Kit== | ==Feature 1: Consumables Kit== | ||
<!-- Instruction 1: Summarize how the consumables (liquid reagents and small plastics) will be packaged in your kit. You may add a schematic image. An image is OPTIONAL and will not get bonus points, but it will make your report look awesome and easy to score. --> | <!-- Instruction 1: Summarize how the consumables (liquid reagents and small plastics) will be packaged in your kit. You may add a schematic image. An image is OPTIONAL and will not get bonus points, but it will make your report look awesome and easy to score. --> | ||
<!-- Instruction 2: IF your consumables packaging plan addresses any major weakness(es), explain how in an additional paragraph. --> | |||
<br> For our new device, the consumable products will come premeasured to ensure accuracy. A set of eight PCR test tubes containing the predetermined 50 μL of PRC mixture (containing the TAQ polymerase) will be provided along with pre-labeled test tubes with 50 μL of DNA. Besides this, the kit will come with the necessary pipette, sterile pipette tips, and gloves. | |||
The inclusion of premeasured consumables is meant to prevent any human error in the PCR process. With the tubes of 50 μL PCR mix, the individual will only have to pipette the DNA samples into the mix and not worry about the amount of PCR mixture. Besides this, the test tubes, both for the primer mixture and DNA are labeled to prevent any confusion or cross contamination. The tubes for both the PCR mixture and DNA will correspond. | |||
==Feature 2: Hardware - PCR Machine & Fluorimeter== | ==Feature 2: Hardware - PCR Machine & Fluorimeter== | ||
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<!-- Instruction 2: IF your group has decided to redesign the PCR machine and/or Fluorimeter to address any major weakness(es), explain how in an additional paragraph. --> | <!-- Instruction 2: IF your group has decided to redesign the PCR machine and/or Fluorimeter to address any major weakness(es), explain how in an additional paragraph. --> | ||
<br> | <br> The consumables will still be processed through a PCR machine to denature the DNA. The fluorimeter will still be used to process the image of the DNA to look for any tell-tale signs of disease. Some adjustments made to the PCR machine include a quicker heating process for the PCR machine. The denaturing process is quite lengthy and the machine takes a while to go from the differing temperatures and on numerous cycles. | ||
The fluorimeter, will come equipped with a built- in camera as well as the stand for the slide that is prepositioned to the correct distance from the camera. This will ensure that the image is taken form the correct location as well as remove potential error from the smartphone camera. Once the images are taken, a USB cord will connect the the fluorimeter to a computer and enter images. A downloadable program will then auto-generate the ImageJ processing, making the process more convenient and time efficient. | |||
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Revision as of 19:40, 25 November 2014
BME 100 Fall 2014 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | ||||||
OUR COMPANY
LAB 6 WRITE-UPBayesian StatisticsOverview of the Original Diagnosis System
Computer-Aided DesignTinkerCAD TinkerCAD is an online product design service that allowed us to replicate the structure of the OpenPCR machine. Using the building blocks given to us through the website thingiverse.com, we re-created a PCR pachine virtually. We then modified this basic structure to incorporate some of the changes we felt would better the device. Our Design
Feature 1: Consumables Kit
The inclusion of premeasured consumables is meant to prevent any human error in the PCR process. With the tubes of 50 μL PCR mix, the individual will only have to pipette the DNA samples into the mix and not worry about the amount of PCR mixture. Besides this, the test tubes, both for the primer mixture and DNA are labeled to prevent any confusion or cross contamination. The tubes for both the PCR mixture and DNA will correspond. Feature 2: Hardware - PCR Machine & Fluorimeter
The fluorimeter, will come equipped with a built- in camera as well as the stand for the slide that is prepositioned to the correct distance from the camera. This will ensure that the image is taken form the correct location as well as remove potential error from the smartphone camera. Once the images are taken, a USB cord will connect the the fluorimeter to a computer and enter images. A downloadable program will then auto-generate the ImageJ processing, making the process more convenient and time efficient. |