OUR TEAM
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LAB 5 WRITE-UP
Procedure
Smart Phone Camera Settings
- Type of Smartphone: Android S4
- Flash: Inactivated
- ISO setting:800
- White Balance: Auto
- Exposure: Highest Setting (+2.0)
- Saturation: Highest Setting
- Contrast:Lowest Setting
Calibration
- Distance between the smart phone cradle and drop =
Solutions Used for Calibration
Initial Concentration of 2X Calf Thymus DNA solution (micrograms/mL) |
Volume of the 2X DNA solution (µL) |
Volume of the SYBR GREEN I Dye solution (µL) |
Final DNA concentration in SYBR Green I solution (µg/mL)
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5 |
80 |
80 |
2.5
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2 |
80 |
80 |
1
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1 |
80 |
80 |
0.5
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0.5 |
80 |
80 |
0.25
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0.25 |
80 |
80 |
0.125
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0 |
80 |
80 |
0
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Placing Samples onto the Fluorimeter
- [Instructions: Step one, in your OWN words]
- [Instructions: Step two, in your own words]
- [Instructions: Step three, in your own words]
- [Instructions: Step etc., in your own words]
Data Analysis
Representative Images of Negative and Positive Samples
Image J Values for All Calibrator Samples
TABLE GOES HERE
Calibration curve
PCR Results Summary
- Our positive control PCR result was ____ μg/mL
- Our negative control PCR result was ____ μg/mL
Observed results
- Patient _____ :
- Patient _____ :
Conclusions
- Patient _____ :
- Patient _____ :
SNP Information & Primer Design
Background: About the Disease SNP
Primer Design and Testing
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