BME100 f2014:Group13 L5

BME 100 Fall 2014	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Katherine Salazar Name: Micheal Nguyen Name: Jacob Irwin Name: Esteban Cruz Name: Dalal Almuhaidib Name: Nicholas Olivar LAB 5 WRITE-UP Procedure Smart Phone Camera Settings Type of Smartphone: Android S4 Flash: Inactivated ISO setting:800 White Balance: Auto Exposure: Highest Setting (+2.0) Saturation: Highest Setting Contrast:Lowest Setting Calibration Adjust the smartphone camera settings to the qualifications above, if possible. Place the phone in the cradle at a right angle from the slide. Adjust the height of the fluorimeter using the plastic trays so that the phone takes is viewing the drop from the side. Change the distance between the smartphone in its cradle and the first two rows of the slide so that it is as close as possible (but further the 4 cm away from the drop) without making the image blurry. Measure and record the distance from the smartphone in the cradle and the drop. Distance between the smart phone cradle and drop = Solutions Used for Calibration Initial Concentration of 2X Calf Thymus DNA solution (micrograms/mL) Volume of the 2X DNA solution (µL) Volume of the SYBR GREEN I Dye solution (µL) Final DNA concentration in SYBR Green I solution (µg/mL) 5 80 80 2.5 2 80 80 1 1 80 80 0.5 0.5 80 80 0.25 0.25 80 80 0.125 0 80 80 0 Placing Samples onto the Fluorimeter [Instructions: Step one, in your OWN words] [Instructions: Step two, in your own words] [Instructions: Step three, in your own words] [Instructions: Step etc., in your own words] Data Analysis Representative Images of Negative and Positive Samples Image J Values for All Calibrator Samples TABLE GOES HERE Calibration curve PCR Results Summary Our positive control PCR result was ____ μg/mL Our negative control PCR result was ____ μg/mL Observed results Patient _____ : Patient _____ : Conclusions Patient _____ : Patient _____ : SNP Information & Primer Design Background: About the Disease SNP Primer Design and Testing