BME100 f2014:Group13 L5

BME 100 Fall 2014	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Katherine Salazar Name: Micheal Nguyen Name: Jacob Irwin Name: Esteban Cruz Name: Dalal Almuhaidib Name: Nicholas Olivar LAB 5 WRITE-UP Procedure Smart Phone Camera Settings Type of Smartphone: Android S4 Flash: Inactivated ISO setting:800 White Balance: Auto Exposure: Highest Setting (+2.0) Saturation: Highest Setting Contrast:Lowest Setting Calibration Distance between the smart phone cradle and drop = Solutions Used for Calibration Initial Concentration of 2X Calf Thymus DNA solution (micrograms/mL) Volume of the 2X DNA solution (µL) Volume of the SYBR GREEN I Dye solution (µL) Final DNA concentration in SYBR Green I solution (µg/mL) 5 80 80 2.5 2 80 80 1 1 80 80 0.5 0.5 80 80 0.25 0.25 80 80 0.125 0 80 80 0 Placing Samples onto the Fluorimeter [Instructions: Step one, in your OWN words] [Instructions: Step two, in your own words] [Instructions: Step three, in your own words] [Instructions: Step etc., in your own words] Data Analysis Representative Images of Negative and Positive Samples Image J Values for All Calibrator Samples TABLE GOES HERE Calibration curve PCR Results Summary Our positive control PCR result was ____ μg/mL Our negative control PCR result was ____ μg/mL Observed results Patient _____ : Patient _____ : Conclusions Patient _____ : Patient _____ : SNP Information & Primer Design Background: About the Disease SNP Primer Design and Testing