BME100 f2014:Group13 L5: Difference between revisions

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{| {{table}} width=700
{| {{table}} width=700
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| Initial Concentration of 2X
| Initial Concentration of 2X Calf Thymus DNA solution (micrograms/mL) || Volume of the 2X DNA solution (µL) || Volume of the SYBR GREEN I Dye solution (µL) || Final DNA concentration in SYBR Green I solution (µg/mL)
Calf Thymus DNA solution
(micrograms/mL) || row 1 cell 2 || row 1 cell 3 || row 1 cell 4
|-
|-
| row 2 cell 1 || row 2 cell 2 || row 2 cell 3 || row 2 cell 4
| row 2 cell 1 || row 2 cell 2 || row 2 cell 3 || row 2 cell 4

Revision as of 09:49, 29 October 2014

BME 100 Fall 2014 Home
People
Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
Course Logistics For Instructors
Photos
Wiki Editing Help


OUR TEAM

Name: Katherine Salazar
Name: Micheal Nguyen
Name: Jacob Irwin
Name: Esteban Cruz
Name: Dalal Almuhaidib
Name: Nicholas Olivar


LAB 5 WRITE-UP

Procedure

Smart Phone Camera Settings

  • Type of Smartphone: Android S4
    • Flash: Inactivated
    • ISO setting:800
    • White Balance: Auto
    • Exposure: Highest Setting (+2.0)
    • Saturation: Highest Setting
    • Contrast:Lowest Setting


Calibration

  • Distance between the smart phone cradle and drop =


Solutions Used for Calibration

Initial Concentration of 2X Calf Thymus DNA solution (micrograms/mL) Volume of the 2X DNA solution (µL) Volume of the SYBR GREEN I Dye solution (µL) Final DNA concentration in SYBR Green I solution (µg/mL)
row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4
row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4



Placing Samples onto the Fluorimeter

  1. [Instructions: Step one, in your OWN words]
  2. [Instructions: Step two, in your own words]
  3. [Instructions: Step three, in your own words]
  4. [Instructions: Step etc., in your own words]


Data Analysis

Representative Images of Negative and Positive Samples


Image J Values for All Calibrator Samples


TABLE GOES HERE


Calibration curve


PCR Results Summary

  • Our positive control PCR result was ____ μg/mL
  • Our negative control PCR result was ____ μg/mL

Observed results

  • Patient _____ :
  • Patient _____ :

Conclusions

  • Patient _____ :
  • Patient _____ :




SNP Information & Primer Design

Background: About the Disease SNP


Primer Design and Testing