BME100 f2013:W900 Group9 L5: Difference between revisions
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'''Placing Samples onto the Fluorimeter''' | '''Placing Samples onto the Fluorimeter''' | ||
# '' | # ''1) Attach fresh tip to micropipette and set micropipette to 50 micro-liters'' | ||
# '' | # ''2) Push micropipette plunger to first stop, and place tip into SYBR Green 1 dye, release plunger'' | ||
# '' | # ''3) Lift tip out of SYBR Green 1 dye and hover micropipette tip directly between two holes of the fluorimeter slide'' | ||
# '' | # ''4) Push plunger down to second stop, allowing dye to release onto the slide'' | ||
# ''5) Repeat steps 1-4 but now with the DNA'' | |||
# ''6) After observing the solution on the fluorimeter, discard the solution'' | |||
# ''7) Push micropipette plunger to first stop, place tip in the solution'' | |||
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Revision as of 22:52, 12 November 2013
BME 100 Fall 2013 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAM
LAB 5 WRITE-UPBackground InformationSYBR Green Dye Single-Drop Fluorimeter How the Fluorescence Technique Works
ProcedureSmart Phone Camera Settings
Solutions Used for Calibration
Data AnalysisRepresentative Images of Samples 0.0 μg/mL DNA Concentration Image J Analysis
Image J Values for All Samples
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