BME100 f2013:W900 Group9 L5: Difference between revisions
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''[Instructions: A description of the single-drop fluorimeter device. Add a PHOTO for bonus points]''<br> | ''[Instructions: A description of the single-drop fluorimeter device. Add a PHOTO for bonus points]''<br> | ||
A single-drop fluorimeter device detects PCR-amplified DNA molecules based on the amount of flourescent material in the sample. A blue LED light is shined upon the sample; a camera can then capture green light flourescened by the DNA. | A single-drop fluorimeter device detects PCR-amplified DNA molecules based on the amount of flourescent material in the sample. A blue LED light is shined upon the sample; a camera can then capture green light flourescened by the DNA. | ||
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[[Image:Group9_Fluorimeter.jpg|450px]] | |||
'''How the Fluorescence Technique Works'''<br> | '''How the Fluorescence Technique Works'''<br> |
Revision as of 20:12, 12 November 2013
BME 100 Fall 2013 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAM
LAB 5 WRITE-UPBackground InformationSYBR Green Dye Single-Drop Fluorimeter How the Fluorescence Technique Works
ProcedureSmart Phone Camera Settings
[Instructions: Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.]
The phone was placed in the cradle at an appropriate angle to view the slide from the side. The fluorimeter was raised in front of the phone to get the right angle. A light box was placed over the set up to provide darkness to take the picture of the light flowing through the DNA and SYBR Green 1. The fluorescence in the sample was easily seen with higher concentrations of SYBR Green 1.
[Instructions: See worksheet page 6.]
Data AnalysisRepresentative Images of Samples [Instructions: Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA]
Image J Values for All Samples
[Instructions: Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 9]
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