BME100 f2013:W900 Group5 L5: Difference between revisions
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*SYBR Green dye works on the principal of molecular bonding, in the way that it bonds with double bonded strains of DNA more that the rest of the weakly copied single strands. | *SYBR Green dye works on the principal of molecular bonding, in the way that it bonds with double bonded strains of DNA more that the rest of the weakly copied single strands. | ||
*There were 6 different DNA's that we have to mix with the Green SYBR, then observe the change of color while mixing the Green SYBR with each DNA separately. | *There were 6 different DNA's that we have to mix with the Green SYBR, then observe the change of color while mixing the Green SYBR with each DNA separately. | ||
'''Single Drop Fluorimeter''' | |||
'''Single Drop Fluorimeter''' | |||
*A Fluorimeter is an instrument that detects fluorescence (a very sensitive detection technique that uses the generation of light by a molecule due to excitation by shining light of a shorter wavelength) quantity | *A Fluorimeter is an instrument that detects fluorescence (a very sensitive detection technique that uses the generation of light by a molecule due to excitation by shining light of a shorter wavelength) quantity | ||
*6 drop trails were conducted for each sample. The Green Syper with different DNA sample ,each time. The change of color was obvious. The color was changing to green. | *6 drop trails were conducted for each sample. The Green Syper with different DNA sample ,each time. The change of color was obvious. The color was changing to green. | ||
''How The Fluoresces Technique Works''' | |||
* The Fluoresces Technique works in various steps. First step that is taken in this process, is the action of the SYBR Green Dye infiltrating the dsDNA and binding to the center of the acid by molecular means. After this is completed the Green dye just sits and waits until it has been excited by an outside force. This force is fulfilled in the way of the blue light emitted from the ''Single Drop Fluorimeter''. The SYBR Green Dye has a lower frequency than the blue light emitted, so the blue acts upon the Green Dye rather than the other way around. This is in turn recorded by a camera phone and analyzed by the ImageJ software. This software provides the most accurate information about light and it's gradients. | * The Fluoresces Technique works in various steps. First step that is taken in this process, is the action of the SYBR Green Dye infiltrating the dsDNA and binding to the center of the acid by molecular means. After this is completed the Green dye just sits and waits until it has been excited by an outside force. This force is fulfilled in the way of the blue light emitted from the ''Single Drop Fluorimeter''. The SYBR Green Dye has a lower frequency than the blue light emitted, so the blue acts upon the Green Dye rather than the other way around. This is in turn recorded by a camera phone and analyzed by the ImageJ software. This software provides the most accurate information about light and it's gradients. | ||
*The Fluorescence Technique Worked perfictly. The color was changing to green gradually everytime we mix one of the DNA's with the Green SYBR. | *The Fluorescence Technique Worked perfictly. The color was changing to green gradually everytime we mix one of the DNA's with the Green SYBR. |
Revision as of 15:21, 6 November 2013
BME 100 Fall 2013 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||
OUR TEAM
LAB 5 WRITE-UPBackground InformationSYBR Green Dye
Single Drop Fluorimeter
How The Fluoresces Technique Works'
ProcedureSmart Phone Camera Settings
[Instructions: Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.]
[Instructions: See worksheet page 6.]
[Add more rows as needed]
Data AnalysisRepresentative Images of Samples
[Instructions: Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 9]
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