BME100 f2013:W900 Group3 L6
BME 100 Fall 2013 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | ||||
OUR COMPANYThermo Diagnostics
LAB 6 WRITE-UPComputer-Aided DesignTinkerCAD TinkerCAD was is a free online tool used to create simple 3D files that can be saved as .stl which can be used in more advanced programs or even 3d printed. On Nov 20th, we used it to quickly mock up an alteration to the concept of the flourimeter, which we thought was a little to big and bulky. Not only did the program allow us to include a visual document in the report, but it also allowed our team members to more effectively communicate the concepts that were difficult to explain in words.
Implications of Using TinkerCAD for Design The redesign of the flourimeter was done using TinkerCAD software. The speed and simplicity at which a 3D technical image can be created allowed for team members to do more in less time. While the mock up wasn't anything that was ready to be printed and utilized as is, it did perfectly illustrate a concept and allow the team to all be on the same page in the redesign. If it was required, it would be possible to create a .stl file with enough precision to 3D print much of the flourimeter turning out a working prototype in less than a day.
Feature 1: Cancer SNP-Specific PrimersBackground on the cancer-associated mutation rs17879961 is a pathogenic mutation found on the 22nd chromosome on the CHEK 2 gene of homo sapiens. SNPs in the CHEK2 gene have been linked to inheritable cancer, as the gene normally produces a protein called checkpoint kinase 2, which functions in tumor suppression.
Primer design
Feature 2: Consumables KitIncluded in the ThermoDiagnostics consumables kit will be all plastics; including the PCR tube holders, pipette tips, and the tube plate, also included is the micropipettor, and reagents. The micropipettor is used to easily ensure accurate measurements when transferring liquids from any tubes. Also added will be labels to our PCR tubes to avoid any confusions and/or mistakes as to which solution it is. To avoid human error in regards to the micropipettor, an instructional DVD will be included in the kit, and the instructions will reiterate the importance of watching this instructional video prior to any use of the micropippetor. This will eliminate any air bubble problems or difficulties obtaining all the liquid materials from the PCR tubes. Because the SYBR green was sensitive to the light, making the results compose of discrepancies overtime we propose that we put the film used on sunglasses and coat it on the outside of the test tube. This would slow down the light that penetrates through the PCR tube and make the results more accurate overtime.
Bonus Opportunity: What Bayesian Stats Imply About The BME100 Diagnostic Approach
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