BME100 f2013:W900 Group1 L4

BME 100 Fall 2013	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Neema Jamali Name: Kaitlyn Allen Name: Riddhi Rohit Name: Carter Hill Name: student Role(s) Name: student Role(s) LAB 1 WRITE-UP Initial Machine Testing The Original Design (Add image of the full OpenPCR machine here, from the Week 9 exercise. Write a paragraph description for visitors who have no idea what this is) Experimenting With the Connections When we unplugged (part 3) from (part 6), the machine ... (did what? fill in your answer) When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer) Test Run (Write the date you first tested Open PCR and your experience(s) with the machine) Protocols Thermal Cycler Program DNA Sample Set-up Positive Control (PC0) Patient ID 67618 Replicate 1 (PC1) Patient ID 67618 Replicate 2 (PC2) Patient ID 67618 Replicate 3 (PC3) Negative Control (NC0) Patient ID 78379 Replicate 1 (NC1) Patient ID 78379 Replicate 2 (NC2) Patient ID 78379 Replicate 3 (NC3) DNA Sample Set-up Procedure Step 1: Label the tubes. Step 2: Add PCR reaction and DNA/ primer mix to test tubes. Step 3: Run machine and collect data. PCR Reaction Mix What is in the PCR reaction mix? PCR reaction, 8 tubes, 50 μL each: Mix contains Taq DNA polymerase, MgCl2, and dNTP's. DNA/ primer mix What is in the DNA/ primer mix? DNA/ primer mix, 8 tubes, 50 μL each: Each mix contains a different template DNA. All tubes have the same forward primer and reverse primer. Research and Development PCR - The Underlying Technology Functions The function of the primers include attaching to sites on the DNA strands that are at either end of the segment that needs to be copied. The taq polymerase reads the DNA code and then attaches matching nucleoties to create DNA copies. Steps of Thermal Cycling During the denature step at 95 degrees C for 30 seconds, the DNA double helix seperates creating two single-stranded DNA molecules. Next, during the anneal stage at 57 degrees C for 30 seconds, a single stranded DNA molecule naturally attempts to pair up. Furthermore, at the extend stage at 72 degrees C for 30 seconds the DNA polymerase is activated and adds complementary nucleotides onto the strand. Lastly. during the final stage at 72 degrees C for 3 minutes there are fragments left that only contain the target sequence of DNA. (Add a write-up, essay-style, organized into paragrpahs with descriptive headers, based on the Q&A's from Section three of your worksheet) (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the PCR video/ tutorial might be useful. Be sure to credit the sources if you borrow images.)