BME100 f2013:W900 Group1 L4: Difference between revisions
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'''DNA Sample Set-up Procedure''' | '''DNA Sample Set-up Procedure''' | ||
# Step 1 | # Step 1. | ||
# Step 2 | # Step 2 | ||
# Step 3... | # Step 3... | ||
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* What is in the PCR reaction mix? | * What is in the PCR reaction mix? | ||
PCR reaction, 8 tubes, 50 μL each: Mix contains Taq DNA polymerase, MgCl2, and dNTP's. | PCR reaction, 8 tubes, 50 μL each: Mix contains Taq DNA polymerase, MgCl2, and dNTP's. | ||
'''DNA/ primer mix''' | '''DNA/ primer mix''' |
Revision as of 10:35, 23 October 2013
BME 100 Fall 2013 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||
OUR TEAMLAB 1 WRITE-UPInitial Machine TestingThe Original Design
When we unplugged (part 3) from (part 6), the machine ... (did what? fill in your answer) When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer)
(Write the date you first tested Open PCR and your experience(s) with the machine)
ProtocolsThermal Cycler Program
PCR reaction, 8 tubes, 50 μL each: Mix contains Taq DNA polymerase, MgCl2, and dNTP's.
DNA/ primer mix, 8 tubes, 50 μL each: Each mix contains a different template DNA. All tubes have the same forward primer and reverse primer.
Research and DevelopmentPCR - The Underlying Technology (Add a write-up, essay-style, organized into paragrpahs with descriptive headers, based on the Q&A's from Section three of your worksheet) (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the PCR video/ tutorial might be useful. Be sure to credit the sources if you borrow images.)
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